Team:Dundee/Sequences

Sequences and Parts

Sequences for the Blood-detector

Detecting Blood

The biobricks in this section are designed for the detection of blood. All sequences are synthetically optimised for expression in our chassis.

Haemoglobin A

>BBa_K1590000 (Haemoglobin A)
ATGGTTCTGTCTCCGGCGGACAAAACCAACGTTAAAGCGGCGTGGGGTAAAGTTGGTGCGCACGCGGGTGAATACGGTGCGGAAGCGCTGGAACGTATGTTCCTGTCTTTCCCGACCACCAAAACCTACTTCCCGCACTTCGACCTGTCTCACGGTTCTGCGCAGGTTAAAGGTCACGGTAAAAAAGTTGCGGACGCGCTGACCAACGCGGTTGCGCACGTTGACGACATGCCGAACGCGCTGTCTGCGCTGTCTGACCTGCACGCGCACAAACTGCGTGTTGACCCGGTTAACTTCAAACTGCTGTCTCACTGCCTGCTGGTTACCCTGGCGGCGCACCTGCCGGCGGAGTTCACCCCGGCGGTTCACGCGTCTCTGGACAAATTCCTGGCGTCTGTTTCTACCGTTCTGACCTCTAAATACCGTTAA

Haemoglobin B

>BBa_K1590001 (Haemoglobin B)
ATGGTTCACCTGACCCCGGAAGAAAAATCTGCGGTTACCGCGCTGTGGGGTAAAGTTAACGTTGACGAAGTTGGTGGTGAAGCGCTGGGTCGTCTGCTGGTTGTTTACCCGTGGACCCAGCGTTTCTTCGAATCTTTCGGTGACCTGTCTACCCCGGACGCGGTTATGGGTAACCCGAAAGTTAAAGCGCACGGTAAAAAAGTTCTGGGTGCGTTCTCTGACGGTCTGGCGCACCTGGACAACCTGAAAGGCACCTTCGCGACCCTGTCTGAACTGCACTGCGACAAACTGCACGTTGACCCGGAAAACTTCCGTCTGCTGGGTAACGTTCTGGTTTGCGTTCTGGCGCACCACTTCGGTAAAGAGTTCACCCCGCCGGTTCAGGCGGCGTACCAGAAAGTTGTTGCGGGTGTTGCGAACGCGCTGGCGCACAAATACCACTAA

Haptoglobin

>BBa_K1590002 (Haptoglobin)
ATGTCTGCGCTGGGTGCGGTTATCGCGCTGCTGCTGTGGGGTCAGCTGTTCGCGGTTGACTCTGGTAACGACGTTACCGACATCGCGGACGACGGTTGCCCGAAACCGCCGGAAATCGCGCACGGTTACGTTGAACACTCTGTTCGTTACCAGTGCAAAAACTACTACAAACTGCGTACCGAAGGTGACGGTGTTTACACCCTGAACGACAAAAAACAGTGGATCAACAAAGCAGTAGGCGACAAACTGCCGGAATGCGAAGCCGATGACGGCTGTCCGAAACCACCGGAGATCGCTCATGGCTACGTCGAACACTCGGTGCGTTATCAGTGCAAAAACTACTACAAACTGCGCACGGAAGGAGACGGGGTGTACACTCTTAATAACGAGAAACAGTGGATTAACAAAGCGGTTGGCGATAAGCTGCCGGAATGTGAGGCAGTCTGCGGTAAACCGAAAAACCCGGCGAACCCGGTTCAGCGTATCTTGGGCGGGCACTTAGATGCGAAAGGTTCATTCCCGTGGCAGGCCAAAATGGTTTCCCACCACAATCTCACGACAGGGGCGACCCTGATCAACGAGCAGTGGTTGCTGACCACTGCTAAAAATCTGTTTCTGAACCATAGCGAAAATGCGACGGCGAAAGACATCGCGCCGACCCTGACCCTGTACGTTGGTAAAAAACAGCTGGTTGAAATCGAAAAAGTTGTTCTGCACCCGAACTACTCTCAGGTTGACATCGGTCTGATCAAACTGAAACAGAAAGTTTCTGTTAACGAACGTGTTATGCCGATCTGCCTGCCGTCTAAAGACTACGCGGAAGTTGGTCGTGTTGGTTACGTTTCTGGTTGGGGTCGTAACGCGAACTTCAAATTCACCGACCACCTGAAATACGTTATGCTGCCGGTTGCGGACCAGGACCAGTGCATCCGTCACTACGAAGGTTCTACCGTTCCGGAAAAAAAAACCCCGAAATCTCCGGTTGGTGTTCAGCCGATCCTGAACGAACACACCTTCTGCGCGGGTATGTCTAAATACCAGGAAGACACCTGCTACGGTGACGCGGGTTCTGCGTTCGCGGTTCACGACCTGGAAGAAGACACCTGGTACGCGACCGGTATCCTGTCTTTCGACAAATCTTGCGCGGTTGCGGAATACGGTGTTTACGTTAAAGTTACCTCTATCCAGGACTGGGTTCAGAAAACCATCGCGGAAAACTAA

Sequences for the Chromate-detector

Detecting Chromate

This set of biobricks is based on an operon of ochrobactrum tritici, conferring resistance against Cr(VI). The Beijing institute of Biotechnology already submitted a biobrick based on this operon in 2013, BBa_K1058008 (See Figure 1). We used this brick, broke it down to its constituent parts, and reassembled it in a different way (See Figure 2).

Figure 1: Plasmid map of BBa_K1058008
by BIT2013.

Figure 2: Plasmid maps of BBa_K1590006 (pChrGFP) and BBa_K1590009 (ChrB).
These 2 biobricks are transformed into one cell in order to rebuild a chromate sensor.

Recommended reading:

• Branco R et al. The Chromate-Inducible chrBACF Operon from the Transposable Element TnOtChr Confers Resistance to Chromium(VI) and Superoxide. Journal of Bacteriology. 2008;190(21):6996-7003.
• Branco R et al. Highly Sensitive, Highly Specific Whole-Cell Bioreporters for the Detection of Chromate in Environmental Samples. PloS one. 2013;8(1):e54005.

pChrGFP by BIT 2013

>BBa_K1058008
ATTGCTTATTCCTATTGCCATTTGCTTTTATTTTGCAATACGTTTTAGCAACGCACAACACCTTATGGGTGTGCTGCTTCCATTGTGATTGCGCAAATGTCCGTTTTTGCAATCTACTCAAGACTTTATTTCGTAGATCTTATCTCATTATTGTAGTAACATCTACGACATGAATTTGCTTTCGCCTATCCTTTCCTTGCCTACTGAAAACGCGACCGTCCGGCAACGGACGTGGCGTGCCCTCAAAGCCTCCGGCGCTGCGGTGCTGCGCGACGGCGTGTACCTGATGCCCGACCGCGACGAATGCCGCGCGGTGCTGGATAACTTGGCCTCCGATGTGCGTGAAGGCGGTGGGGTCGCTCATGTGCTGCGTATGGAAGACCCTGAAGGGGTCAACTTCGTCGCATTATTCGACCGCAGCAACGATTTTGCTGCCTTGCTGGTCGATGTCCATCATCTCAGGCAGACGCTGACATTGGATACCGTGCAAGACGTGCTGCGGCAGGTGCGTAAGCTTCGCAAATCCTTTACTACGCTGGTTGAAATCGACTTCTACCCCGGCGAGGCTCAGCGTCAAGCGGACAGTGCGTTGTGTGAACTGGAACAAGCTTGCGCCCGCACGCTGTCGCCGGACGAGCCGCACGCTGTAGAGGGGACTATTACCCGCTTGGATCGTTTGGATTACCAGGCCCGTACCTGGGCCACTCGCGCACGTCCTTGGGTTGATCGGCTCGCCAGCGCATGGCTGATCCGGCGCTTCATCGACCCGCAGGCGCGCATCCTTTGGTTGGCAACCCCTGCGGACTGCCCGCCGGATGCGTTGGGCTTCGACTTCGATGGCGCGACGTTCAGCCATGTCGGCAGCCGTGTCACGTTCGAGGTCCTGGCGGCGAGCTTTGGGCTGGAACAGCCCGCCATCACAAGGATTGGCCTTGTGGTGCATTACCTCGACGTGGGCGGCATCCAGCCGCCAGAGGCCACTGGTATCGAAAGCGTACTGGCCGGTTTGCGGGAAACGGTTGACCACGACGATCAACTATTGGCCATCGCCTCCACTGTGTTCGATGGCTTACTGGCCAGCTTTGAAAAAGGGACCCTCACCGTATGAGGATCCGAAGGAGATATACCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTA

pChr

>BBa_K1590003 (pChr)
ATTGCTTATTCCTATTGCCATTTGCTTTTATTTTGCAATACGTTTTAGCAACGCACAACACCTTATGGGTGTGCTGCTTCCATTGTGATTGCGCAAATGTCCGTTTTTGCAATCTACTCAAGACTTTATTTCGTAGATCTTATCTCATTATTGTAGTAACATCTACGAC

ChrB

>BBa_K1590004 (ChrB)
ATGAATTTGCTTTCGCCTATCCTTTCCTTGCCTACTGAAAACGCGACCGTCCGGCAACGGACGTGGCGTGCCCTCAAAGCCTCCGGCGCTGCGGTGCTGCGCGACGGCGTGTACCTGATGCCCGACCGCGACGAATGCCGCGCGGTGCTGGATAACTTGGCCTCCGATGTGCGTGAAGGCGGTGGGGTCGCTCATGTGCTGCGTATGGAAGACCCTGAAGGGGTCAACTTCGTCGCATTATTCGACCGCAGCAACGATTTTGCTGCCTTGCTGGTCGATGTCCATCATCTCAGGCAGACGCTGACATTGGATACCGTGCAAGACGTGCTGCGGCAGGTGCGTAAGCTTCGCAAATCCTTTACTACGCTGGTTGAAATCGACTTCTACCCCGGCGAGGCTCAGCGTCAAGCGGACAGTGCGTTGTGTGAACTGGAACAAGCTTGCGCCCGCACGCTGTCGCCGGACGAGCCGCACGCTGTAGAGGGGACTATTACCCGCTTGGATCGTTTGGATTACCAGGCCCGTACCTGGGCCACTCGCGCACGTCCTTGGGTTGATCGGCTCGCCAGCGCATGGCTGATCCGGCGCTTCATCGACCCGCAGGCGCGCATCCTTTGGTTGGCAACCCCTGCGGACTGCCCGCCGGATGCGTTGGGCTTCGACTTCGATGGCGCGACGTTCAGCCATGTCGGCAGCCGTGTCACGTTCGAGGTCCTGGCGGCGAGCTTTGGGCTGGAACAGCCCGCCATCACAAGGATTGGCCTTGTGGTGCATTACCTCGACGTGGGCGGCATCCAGCCGCCAGAGGCCACTGGTATCGAAAGCGTACTGGCCGGTTTGCGGGAAACGGTTGACCACGACGATCAACTATTGGCCATCGCCTCCACTGTGTTCGATGGCTTACTGGCCAGCTTTGAAAAAGGGACCCTCACCGTATGA

Optimised ChrB

>BBa_K1590005 (Partially optimised ChrB)
ATGAACCTGCTGTCTCCGATCCTGTCTCTGCCGACCGAAAACGCGACCGTCCGGCAACGGACGTGGCGTGCCCTCAAAGCCTCCGGCGCTGCGGTGCTGCGCGACGGCGTGTACCTGATGCCCGACCGCGACGAATGCCGCGCGGTGCTGGATAACTTGGCCTCCGATGTGCGTGAAGGCGGTGGGGTCGCTCATGTGCTGCGTATGGAAGACCCTGAAGGGGTCAACTTCGTCGCATTATTCGACCGCAGCAACGATTTTGCTGCCTTGCTGGTCGATGTCCATCATCTCAGGCAGACGCTGACATTGGATACCGTGCAAGACGTGCTGCGGCAGGTGCGTAAGCTTCGCAAATCCTTTACTACGCTGGTTGAAATCGACTTCTACCCCGGCGAGGCTCAGCGTCAAGCGGACAGTGCGTTGTGTGAACTGGAACAAGCTTGCGCCCGCACGCTGTCGCCGGACGAGCCGCACGCTGTAGAGGGGACTATTACCCGCTTGGATCGTTTGGATTACCAGGCCCGTACCTGGGCCACTCGCGCACGTCCTTGGGTTGATCGGCTCGCCAGCGCATGGCTGATCCGGCGCTTCATCGACCCGCAGGCGCGCATCCTTTGGTTGGCAACCCCTGCGGACTGCCCGCCGGATGCGTTGGGCTTCGACTTCGATGGCGCGACGTTCAGCCATGTCGGCAGCCGTGTCACGTTCGAGGTCCTGGCGGCGAGCTTTGGGCTGGAACAGCCCGCCATCACAAGGATTGGCCTTGTGGTGCATTACCTCGACGTGGGCGGCATCCAGCCGCCAGAGGCCACTGGTATCGAAAGCGTACTGGCCGGTTTGCGGGAAACGGTTGACCACGACGATCAACTATTGGCCATCGCCTCCACTGTGTTCGATGGCTTACTGGCCAGCTTTGAAAAAGGGACCCTCACCGTATGA

GFPmut2

>GFPmut2
ATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGCGATGTTAATGGGCAAAAATTCTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGGAAGCTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGCGTATGGTCTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTACAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAAATGGAATACAACTATAACTCACATAATGTATACATCATGGCAGACAAACCAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTAAAGATGGAAGCGTTCAATTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCCAAAGATCCCAACGAAAAGAGAGATCACATGATCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAA

pChrGFP

>BBa_K1590006 (pChrGFP)
ATTGCTTATTCCTATTGCCATTTGCTTTTATTTTGCAATACGTTTTAGCAACGCACAACACCTTATGGGTGTGCTGCTTCCATTGTGATTGCGCAAATGTCCGTTTTTGCAATCTACTCAAGACTTTATTTCGTAGATCTTATCTCATTATTGTAGTAACATCTACGACTACTAGACACAGAGGAACAGGTATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGCGATGTTAATGGGCAAAAATTCTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGGAAGCTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGCGTATGGTCTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTACAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAAATGGAATACAACTATAACTCACATAATGTATACATCATGGCAGACAAACCAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTAAAGATGGAAGCGTTCAATTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCCAAAGATCCCAACGAAAAGAGAGATCACATGATCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAA

Sequences for the Fingerprint-aging device

Lanosterol Synthase

>BBa_K1590007 (Lanosterol_synthase)
GCGCGAATTCGCGGCCGCTTCTAGATGACCGAAGGAACCTGCCTGCGTCGTCGTGGTGGTCCGTACAAAACCGAACCGGCGACCGACCTGGGTCGTTGGCGTCTGAACTGCGAACGTGGTCGTCAGACCTGGACCTACCTACAGGACGAACGTGCGGGTCGTGAACAGACCGGTCTGGAAGCGTACGCGCTGGGTCTGGACACCAAAAACTACTTCAAAGACCTGCCGAAAGCGCACACCGCGTTCGAAGGTGCGCTGAACGGTATGACCTTCTACGTTGGTCTACAGGCGGAAGACGGTCACTGGACCGGTGACTACGGTGGTCCGCTGTTCCTGCTGCCGGGTCTGCTGATCACCTGCCACGTTGCGCGTATCCCGCTGCCAGCTGGTTACCGAGAGGAAATTGTCCGATATCTACGATCAGTACAACTGCCGGACGGTGGTTGGGGTCTGCACATCGAAGACAAATCTACCGTTTTCGGAACCGCGCTGAACTACGTTTCTCTGCGTATCCTGGGTGTTGGTCCGGACGACCCGGACCTGGTTCGTGCGCGTAACATCCTGCACAAAAAAGGTGGTGCGGTTGCGATCCCGTCTTGGGGTAAATTCTGGCTGGCGGTTCTGAACGTTTACTCTTGGGAAGGTCTGAACACCCTGTTCCCGGAAATGTGGCTGTTCCCGGACTGGGCGCCGGCGCACCCGTCTACCCTGTGGTGCCACTGCCGTCAGGTTTACCTGCCGATGTCTTACTGCTACGCGGTTCGTCTGTCTGCGGCGGAAGACCCGCTGGTTCAGTCTCTGCGTCAGGAACTGTACGTTGAAGACTTCGCGTCTATAGATTGGCTAGCACAACGTAACAATGTTGCTCCAGATGAACTGTACACCCCGCACTCTTGGCTGCTGCGTGTTGTTTACGCGCTGCTGAACCTGTACGAACACCACCACTCTGCGCACCTGCGTCAGCGTGCGGTTCAGAAACTGTACGAACACATCGTTGCGGACGACCGTTTCACCAAATCTATCTCTATCGGTCCGATCTCTAAAACCATCAACATGCTGGTTCGTTGGTACGTTGACGGTCCGGCGTCTACCGCGTTCCAGGAACACGTTTCTCGTATCCCGGACTACCTGTGGATGGGTCTGGACGGTATGAAAATGCAGGGCACCAACGGTTCTCAGATATGGGACACCGCGTTCGCGATCCAGGCGCTGCTGGAAGCGGGTGGTCACCACCGTCCGGAGTTCTCTTCTTGCCTACAGAAAGCGCACGAGTTCCTGCGTCTGTCTCAGGTTCCAGATAATCCACCTGATTACCAGAAATACTACCGTCAGATGCGTAAAGGTGGTTTCTCTTTCTCTACCCTGGACTGCGGTTGGATCGTTTCTGACTGCACCGCGGAAGCGCTGAAAGCGGTTCTGCTGCTACAGGAAAAATGCCCGCACGTTACCGAACACATCCCGCGTGAACGTCTGTGCGACGCGGTTGCGGTTCTGCTGAACATGCGTAACCCGGACGGTGGTTTCGCGACCTACGAAACCAAACGTGGTGGTCACCTGCTGGAACTGCTGAACCCGTCTGAAGTTTTCGGTGACATCATGATCGACTACACCTATGTTGAATGCACCAGTGCGGTGATGCAGGCATTAAAATACTTCCACAAGCGCTTTCCGGAACACCGCGCAGCGGAAATCCGCGAAACGTTGACCCAAGGTCTGGAGTTCTGCCGTCGTCAGCAGCGTGCGGACGGTTCTTGGGAAGGTTCTTGGGGTGTTTGCTTCACCTACGGCACCTGGTTCGGTCTGGAAGCGTTCGCGTGCATGGGTCAGACCTACCGTGACGGAACCGCGTGCGCGGAAGTTTCTCGTGCGTGCGACTTCCTGCTGTCTCGTCAGATGGCGGACGGTGGTTGGGGTGAAGACTTCGAATCTTGCGAAGAACGTCGTTACCTACAGTCTGCGCAGTCTCAGATCCACAACACCTGCTGGGCGATGATGGGTCTGATGGCGGTTCGTCACCCGGACATCGAAGCGCAGGAACGTGGTGTTCGTTGCCTGCTGGAAAAACAGCTGCCGAACGGTGACTGGCCGCAGGAAAACATCGCGGGTGTTTTCAACAAATCTTGCGCGATCTCTTACACCTCTTACCGTAACATCTTCCCGATCTGGGCGCTGGGTCGTTTCTCTCAGCTGTACCCGGAACGTGCGCTGGCGGGTCACCCGTAATAATACTAGTAGCGGCCGCTGCAGGCGC

Sequences for the Nasal mucus detector

Odorant Binding Protein

>BBa_K1590008 (Odorant Binding Protein 2A)
ATGCTGTCTTTCACCCTGGAAGAAGAAGACATCACCGGCACCTGGTACGTTAAAGCGATGGTTGTTGACAAAGACTTCCCGGAAGACCGTCGTCCGCGTAAAGTTTCTCCGGTTAAAGTTACCGCGCTGGGTGGTGGTAACCTGGAAGCGACCTTCACCTTCATGCGTGAAGACCGTTGCATCCAGAAAAAAATCCTGATGCGTAAAACCGAAGAACCGGGTAAATTCTCTGCGTACGGTGGTCGTAAACTGATCTACCTGCAAGAACTGCCGGGCACCGACGACTACGTTTTCTACTGCAAAGACCAGCGTCGTGGTGGTCTGCGTTACATGGGTAAACTGGTTGGTCGTAACCCGAACACCAACCTGGAAGCGCTGGAAGAATTTAAAAAACTGGTTCAGCACAAAGGTCTGTCTGAAGAAGACATCTTCATGCCGCTGCAAACCGGTTCTTGCGTTCTGGAACAC

Odorant Binding Protein - Barrel

>BBa_K1590009 (Odorant Binding Protein 2A - Barrel)
ATGCTGTCTTTCACCCTGGAAGAAGAAGACATCACCGGCACCTGGTACGTTAAAGCGATGGTTGTTGACAAAGACTTCCCGGAAGACCGTCGTCCGCGTAAAGTTTCTCCGGTTAAAGTTACCGCGCTGGGTGGTGGTAACCTGGAAGCGACCTTCACCTTCATGCGTGAAGACCGTTGCATCCAGAAAAAAATCCTGATGCGTAAAACCGAAGAACCGGGTAAATTCTCTGCGTACGGTGGTCGTAAACTGATCTACCTGCAAGAACTGCCGGGCACCGACGACTACGTTTTCTACTGCAAAGACCAGCGTCGTGGTGGTCTGCGTTACATGGGTAAACTGGTTGGTCGTAAC

Odorant Binding Protein - Helix

>BBa_K1590010 (Odorant Binding Protein 2A - Helix)
CCGAACACCAACCTGGAAGCGCTGGAAGAATTTAAAAAACTGGTTCAGCACAAAGGTCTGTCTGAAGAAGACATCTTCATGCCGCTGCAAACCGGTTCTTGCGTTCTGGAACAC

Sequences for the Saliva Detector

Lactoferrin Binding Protein A

>BBa_K1590011 (Lactoferrin Binding Protein A)
ATGAATAAGAAACACGGTTTTCCGCTGACTCTGACTGCCTTGGCCATTGCAACCGCTTTTCCGGCTTATGCTGCCCAAGCGGGGGGGGCGACACCTGATGCCGCGCAGACCCAATCCCTGAAAGAGATTACCGTCCGTGCCGCCAAAGTGGGACGGCGATCGAAAGAGGCGACCGGTTTGGGCAAAATCGTCAAAACGTCGGAAACGTTGAACAAAGAACAGGTACTCGGTATCCGCGACCTGACGCGCTACGATCCGGGCGTGGCGGTTGTCGAACAGGGCAACGGCGCGAGCGGCGGCTACTCGATACGCGGCGTGGATAAAAACCGTGTGGCGGTTTCGGTCGACGGTGTTGCCCAAATACAGGCGTTTACCGTGCAGGGATCGTTGAGCGGATACGGCGGACGCGGCGGCAGCGGTGCAATCAACGAAATCGAATATGAAAACATCAGCACGGTGGAAATCGACAAAGGTGCCGGTTCGTCCGATCACGGCAGCGGCGCACTCGGCGGCGCGGTCGCCTTCCGCACCAAAGAGGCGGCAGACCTGATTTCAGACGGCAAAAGTTGGGGGATACAGGCAAAAACCGCCTACGGCAGTAAAAACCGCCAATTTATGAAGTCGCTCGGCGCGGGGTTCAGCAAAGACGGTTGGGAAGGGCTGTTAATCCGAACCGAACGCCAAGGGCGGGAAACGCGCCCGCACGGCGATATTGCGGACGGGGTGGAATACGGCATAGACCGTTTGGACGCGTTCCGTCAGACATACGATATTAAACGCAAGACAAGAGAGCCATTTTTCTCAGTAGAGGGCGAGCGTGAATCCAAGCCCGTGGCAAAATTGGCGGGCTACGGGAAATATTTGAACAACCAGCTCAACCGCTGGGTAAAAGAACGTATTGAACAAAATCAGCCTTTAAGTGCTGAAGAAGAGGCGCAGGTGCGGGAGGCGCAGGCGCGCCACGAAAATCTGTCCGCCCAAGCTTACACGGGCGGCGGCAGGATATTGCCCGATCCGATGGATTACCGCAGCGGCTCTTGGCTTGCCAAGCTGGGCTACCGCTTCGGCGGCAGGCATTATGTCGGCGGCGTGTTTGAGGATACCAAACAGCGTTACGATATCCGCGATATGACGGAAAAACAGTATTACGGTACGGACGAGGCGGAAAAGTTTAGAGACAAGAGCGGGGTGTACGACGGCGACGATTTCCGCGACGGCTTGTATTTTGTGCCGAATATAGAAGAGTGGAAGGGCGATAAAAATTTGGTCAGGGGCATAGGTTTGAAATATTCCCGCACCAAATTTATTGACGAACATCACCGCCGCCGCCGTATGGGTTTGCTGTATCGTTATGAAAACGAAGCGTATTCTGACAATTGGGCGGATAAGGCGGTGTTGTCGTTTGACAAACAGGGCGTGGCAACCGATAACAACACGCTGAAGCTGAATTGCGCCGTGTATCCTGCTGTGGACAAATCCTGCCGCGCGTCGGCGGACAAACCGTATTCCTACGACAGCAGCGACCGTTTCCACTACCGCGAACAGCACAATGTTTTGAATGCCTCGTTTGAGAAATCGCTGAAAAACAAATGGACGAAACACCATCTGACTTTGGGCTTCGGTTACGATGCTTCCAAAGCGATTTCCCGCCCCGAACAGCTTTCCCACAATGCGGCAAGGATTTCGGAATCCACGGGATTCGATGAAAACAATCAAGATAAGTATCTTTTGGGTAAGCCCGAAGTCGTCGAAGGGTCGGTCTGCGGCTACATCGAAACCCTGCGTTCCCGCAAATGCGTGCCAAGAAAAATCAACGGCAGCAATATCCATATTTCTTTGAACGACCGTTTTTCAATCGGCAAATATTTCGATTTCAGCTTGGGCGGCAGGTACGACCGGAAAAACTTCACCACGTCGGAAGAACTCGTCCGCAGCGGGCGGTATGTTGACCGTTCGTGGAACAGCGGCATCTTGTTCAAACCGAACCGGCATTTTTCCGTGTCTTACCGTGCCTCCAGCGGCTTCAGAACGCCCTCATTCCAAGAACTTTTCGGGATAGACATTTATCACGATTATCCGAAAGGCTGGCAGCGTCCCGCCCTGAAATCGGAAAAGGCAGCCAACCGGGAAATCGGTTTGCAGTGGAAGGGCGATTTCGGCTTTTTGGAAATCAGCAGCTTCCGCAACCGTTATACCGATATGATTGCCGTTGCCGATCACAAAACCAAATTGCCGAATCAGGCAGGACAATTGACAGAGATTGATATACGCGATTATTACAATGCCCAAAATATGTCGCTTCAAGGCGTTAATATATTGGGAAAAATCGACTGGAACGGCGTGTATGGCAAACTGCCCGAAGGTTTGTACACCACATTGGCGTACAACCGCATCAAACCGAAATCGGTATCCAACCGGCCGGGACTGTCCCTCCGCAGCTATGCTTTGGATGCGGTACAGCCGTCGCGTTATGTTTTGGGGTTCGGATACGACCAGCCTGAGGGGAAATGGGGCGCAAACATTATGCTGACCTATTCCAAAGGGAAAAACCCTGACGAGCTTGCTTATCTGGCAGGCGATCAAAAACGATATTCGACAAAAAGAGCGTCGTCTTCTTGGTCGACGGCAGACGTTTCCGCCTATCTGAATCTGAAAAAACGGCTGACCTTGAGGGCGGCTATCTACAATATCGGCAACTACCGCTACGTTACTTGGGAATCCTTGCGCCAGACTGCGGAAAGCACGGCAAACCGGCACGGCGGCGACAGCAACTATGGAAGGTATGCCGCACCGGGCAGGAACTTCAGTCTCGCGCTCGAAATGAAGTTTTAA

Sequences for the Saliva Detector

PotD (Spermidine Binding Protein)

>BBa_K1590012 (PotD)
ATGAAAAAATGGTCACGCCACCTGCTCGCGGCGGGTGCTCTGGCACTGGGCATGAGCGCCGCTCACGCCGATGACAACAACACGCTGTATTTCTACAACTGGACCGAGTACGTGCCGCCAGGACTGCTTGAACAGTTCACCAAAGAAACCGGTATTAAGGTTATCTATTCGACTTACGAGTCGAACGAAACCATGTACGCCAAGCTGAAAACATACAAAGACGGTGCCTATGATCTGGTGGTTCCTTCAACCTATTACGTTGATAAAATGCGTAAAGAAGGGATGATCCAGAAGATCGACAAGTCGAAGTTAACAAACTTCAGCAATCTCGATCCAGACATGCTCAACAAGCCTTTTGACCCGAATAACGACTACTCCATTCCGTATATCTGGGGTGCGACGGCGATTGGTGTTAACGGTGATGCGGTGGATCCGAAATCTGTCACCAGCTGGGCCGATCTGTGGAAGCCAGAGTACAAAGGCAGCCTGCTGTTGACCGACGATGCCCGTGAAGTGTTCCAGATGGCGCTGCGTAAGCTGGGCTACTCCGGTAACACCACCGATCCGAAAGAGATTGAAGCTGCATATAACGAGCTGAAAAAACTGATGCCAAACGTGGCAGCCTTTAACTCCGATAACCCGGCGAACCCGTACATGGAAGGCGAAGTTAACCTCGGCATGATCTGGAACGGTTCTGCTTTCGTTGCACGCCAGGCGGGTACGCCAATTGACGTGGTGTGGCCGAAAGAAGGCGGCATTTTCTGGATGGACAGCCTGGCGATCCCGGCAAATGCCAAAAACAAAGAAGGCGCGCTGAAATTGATCAACTTCCTGCTGCGCCCGGATGTGGCAAAACAGGTTGCTGAAACTATCGGTTATCCAACGCCAAACCTTGCGGCGCGTAAGCTGTTAAGTCCAGAAGTGGCGAACGATAAAACACTCTACCCGGATGCTGAAACCATTAAAAATGGCGAATGGCAGAATGACGTTGGCGCAGCCAGCAGCATTTATGAAGAGTATTATCAGAAGCTGAAAGCAGGACGTTAA

Spermine Binding Protein

>BBa_K1590013 (Spermine Binding Protein)
ATGCTGCTGCTGCTGACCCTGGCGTTCCTGGCGTCTCCGACCTGCCGTGCGCAGAACGTTCTGGGTAACGCGGCGGGTAAATACTTCTACGTTCAGGGTGAAGACCAGGGTCAGCTGAAAGGTATGCGTATCTTCCTGTCTGTTTTCAAATTCATCAAAGGTTTCCAGCTCCAGTTCGGTTCTAACTGGACCGACGTTTACGGCACCCGTTCTGACAACTTCATCGACTTCCTGCTGGAAGACGGTGAACACGTTATCAAAGTTGAAGGTTCTGCGGTTATCTGCCTGACCTCTCTGACCTTCACCACCAACAAAGGTCGTGTTGCGACCTTCGGTGTTCGTCGTGGTCGTTACTTCTCTGACACCGGTGGTTCTGACAAACACCTGGTTACCGTTAACGGTATGCACGCGCCGGGTCTGTGCGTTCGTGGTATCGGTTTCAAATGGGGTAACATCAACGCGAACGGTAACGACCACTACAACAACAAAGAAGACAAAGCGGACAACAAAGACGCGGACAACAAAGACGCTGACAATAAAGACGATGGCGATGAGGATGATGATGGAAACGATGACGATGATCAGAAGGACGAATCTTAA

Vectors used throughout the project

pSB1C3

pQE-80L

pUT18

pT25

pUniprom

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