Team:Bielefeld-CeBiTec/Project/PRIA

PRIA

Plasmid-Repressor-Interaction Assay

Introduction

Our second approach to cell free detection of analytes is an approach based on an immobilized repressor that binds to a plasmid. When the analyte binds the repressor, it changes its conformation and releases the DNA. This release is to be measured. We called this approach Plasmid Repressor Interaction Assay. The advantage over the cell extract is, that this approach does not required transcription or translation of a reporter protein and therefore the signal can be measured much faster. Furthermore, it works with just two purified components, thereby further minimizing the risk of releasing GMOs into the wild.

Aim

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Outview

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References

Araújo et al., 2012, Activated Paper Surfaces for the rapid hybridization of DNA through Capillary Transport