Team:ETH Zurich/Part Collection
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Overview
Our whole cancer sensor depends on the sensitivity of the promoter regulated by LldR. LldR is a repressor that binds to O1 and O2 and forms a loop, avoiding any further transcription. If the promoter is too strong it will supress the transcription even in the presene of lactate, which will result in a reduced sensitivity of our system. If the promoter is too weak, it can result in an increased number of false positives. To decide the optimal arrangement of our regulatory system, we designed n promoters by rearranging O1, O2 and a promoter.
Regulatory system design |
BioBrick |
Comments |
| lldRO1-plldR-lldRO2 | BBa_K822000 | |
| J23100-lldRO1-lldRO2 | ||
| J23100-lldRO1-R2oDNA-lldRO2 | ||
| lldRO1-J23100-lldRO2 | ||
| J23117-lldRO1-lldRO2 | ||
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| lldRO1-J23117-lldRO2 | ||
| J23118-lldRO1-lldRO2 | ||
| J23118-lldRO1-R2oDNA-lldRO2 | ||
| lldRO1-J23118-lldRO2 |