Team:ETH Zurich/Parts
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Overview
For completing our project, we required of many parts. Some of these parts where used from the Registry, others were created for this project. In the following section, you can see these parts.
Registry parts
Part Name |
Description |
Registry Number |
| INP-EYFP | Fusion of Ice Nucleation Protein (INP) and Enhanced Yellow Fluorescent Protein (EYFP) | BBa_K523013 |
| cI | cI repressor from E. coli phage lambda modified with an LVA tail for rapid degradation of the protein | BBa_C0051 |
| lacI + LVA | lacI repressor from E. coli (+LVA) | BBa_C0012 |
| HylA | HlyA-tag+Secretion system, allows a protein to be secreted by means of the alpha-hemolysin secretion system in E. coli | BBa_K1166002 |
| pLuxR | Promoter activated by the LuxR protein complexed with the autoinducer homoserine lactone (HSL) | BBa_R0062 |
| pLldR operon | lldPRD operon promoter + RBS from E. coli. In the absence of L-lactate, lldR binds to two operator sites O1 and O2 in the promoter region and inhibits expression | BBa_K822000 |
| InterLab 1 strong promoter | Anderson promoter J23101 from the Anderson collection | BBa_K823005 (BBa_J23101) |
| InterLab 2 medium-strong promoter | Anderson promoter J23106 from the Anderson collection | BBa_K823008 (BBa_J23106) |
| terminator | Transcription terminator for the E.coli RNA polymerase | BBa_B0012 |
| double terminator | Double terminator consisting of BBa_B0010 and BBa_B0012 | BBa_B0015 |
| very strong promoter | Strong member of the family of promoters J23100 through J23119 | BBa_J23100 |
| gfp (InterLab) | intermediate in screening plasmid construction containing GFP | BBa_I13504 |
| medium promoter | Medium member of the family of promoters J23100 through J23119 | BBa_J23118 |
| aiiA | autoinducer inactivation enzyme aiiA (no LVA, an enzyme that inactivates the acylhomoserine lactone (AHL) quorum-sensing signal | BBa_C0160 |
| LuxI | autoinducer synthetase for acylhomoserine lactone (AHL), no LVA | BBa_C0161 |
| InterLab 3 weak promoter | Anderson promoter J23117 from the Anderson collection | BBa_K823013 (BBa_J23117) |
| promoter medium-weak | Medium-weak member of the family of promoters J23100 through J23119 | BBa_J23114 |
| RBS | RBS.3 (medium) derivative of BBa_0030 | BBa_B0032 |
| promoter plus GFP (InterLab control) | J23151 inserted in the Promoter MeasKit | BBa_I20270 |
| terminator | Artificial terminator, estimated %T~>90% | BBa_B1006 |
New constructs
Part Name |
Description |
Registry Number |
| Plasmid 3 | Combination of lldR operator sites O1 and O2 and promoters of variable potency. Gene expression is blocked by LldR protein (expressed separately) and induced upon addition of L-lactate. This was tested with a fusion to GFP. | |
| PL3a | lldRO1-plldR-lldRO2 | |
| PL3b | J23100-lldRO1-lldRO2 | |
| PL3c | J23100-lldRO1-R2oDNA-lldRO2 | |
| PL3d | J23117-lldRO1-lldRO2 | |
| PL3e | J23117-lldRO1-R2oDNA-lldRO2 | |
| PL3f | J23118-lldRO1-lldRO2 | |
| PL3g | J23118-lldRO1-R2oDNA-lldRO2 | |
| PL3h | lldRO1-J23100-lldRO2 | |
| PL3i | lldRO1-J23117-lldRO2 | |
| PL3j | lldRO1-J23118-lldRO2 | |
| PL3k | lldRO1-R2oDNA-lldRO2 |