Team:HSNU-TAIPEI/Project/Benzo a pyrene
Benzo[a]pyrene
✡ Introduction
(1)Why do we detect benzo[a]pyrene?
Because when food,such as fried food ,which is heated above 300 degrees
celcius,food will release benzo[a]pyrene recycled oil usually contain
benzo[a]pyrene[1].
(2)Harm?
Pathway:from skin,breathing in,eating Carcinogenic,environmental
pollution, Skin irritation,eye irritation[1].
(3)Taiwanese regulation
[2](4)National regulation
Europe:The same to Taiwanese regulation[2]
American:The MCL has been set at 0.2 ppb[3]
✡ Circuit Design
So far,we don’t find the protein which can combine with the Benzo[a]pyrene,so first
we must degradate it by Laccase. And its product is Bap-1,6-quinone which can
combine with the protein,in order that we can detect Benzo[a]pyrene. [4]
We put the gene fragment which can produce the Laccase in the E.coli
Let it produce itself.
QsrR is a protein which can combine with Bap-1,6-quinone.[5]As Laccase,we put the
gene fragment in the E.coli.Let it produce itself.
✡ Result
✡ Reference
(註1)Metabolism of mercury, administered as methylmercuric chloride or mercuric chloride, by lactating ruminants.
(註2)台北醫學大學公共衛生學系碩士班碩士論文Graduate Institute.
(註3)Brown, N. L., J. V. Stoyanov, et al. (2003). "The MerR family of transcriptional regulators." FEMS Microbiol Rev 27(2-3): 145-163.
(註4)Park, S. J., J. Wireman, et al. (1992). "Genetic analysis of the Tn21 mer operator-promoter." J Bacteriol 174(7): 2160-2171.