We constructed 5 plasmids [positive control, negative control, device1~3] by using BioBricks and then transformed to E. coli K-12 JM109. We checked them by colony direct PCR and plasmids sequence. We cultivated correct bacterias by shaking until OD660:0.5. Cultivated mediums were transferred to 96-well plate by 100mL. We read the absorbance.
Setting of plate reader is following:
Result and discussion
Definition
We expressed absorbance as relative value for positive control. This expression is following:
Sample : Absorbance in sample
NCav : Average of absorbance in negative control
PCav : Average of absorbance in positive control
Value
Relative absorbance values for positive control are shown below.
