Team:ETH Zurich/Chip
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Chip Design
Introduction
One of the biggest challenges of circulating tumor cells is their scarcity in the blood of patients. To overcome this problem, our first idea was to develop a microfluidic chip in order to perform single cell analysis. The biggest advantage of using a microfluidic chip is its ability to perform high-throughput cell biology. In order to do so, we wanted to produce water-in-oil emulsion droplets, that can then be sorted by a machine analogous to FACS, (inspired from link paper). In the droplets, a mixture of bacteria and mammalian cells would be present. And the bacteria would express the green fluorescent protein only in the presence of cancer cells, exhibiting both increased lactate production rate and sensitivity to sTRAIL.
First Design
However due to the complexity of this setup, we decided to first explore another design