Team:ETH Zurich/Materials
- Project
- Modeling
- Lab
- Human
Practices - Parts
- About Us
Materials
Buffers and media
SOC medium
- 20 g/L Tryptophane
- 5 g/L Yeast extract
- 0.5 g/L NaCl
- 250 mM KCl
- 1M MgCl2
- 50% (w/v) sterile glucose
- Filter the solution through a 0.2 µm filter.
3T3 medium
- 500 mL D-MEM 1x
- 50 mL FBS 100%
- 5 mL GlutaMax 100x
- 5 mL Penicillin (10k units/mL) /Streptomyocin (10k µg/mL )
Chemicals and Kits
Plasmid Purification
- Product: ZR Plasmid Miniprep ™-Classic
- Company: Zymo Research
- Lot: ZRC182892
- Catalog No: D4016
Changes to the protocol included an extra step to dry the column before elution and the use of water for elution instead of elution buffer. For elution of large plasmids we used prewarmed water.
Gel extraction
- Product: ZymocleanTM Gel DNA Recovery Kit
- Company: Zymo Research
- Lot: ZRC183055
- Catalog No: D4002
Changes to the protocol included an extra step to dry the column before elution and the use of water for elution instead of elution buffer. For elution of large plasmids we used prewarmed water.
Bacterial strains and Mammalian Cell Lines
TOP10
The bacterial strain we used as a base for all our transformations was TOP10.
Jurkat Cells
HL60
3T3
SK-BR-3
Machines
Nano drop
Thermo Scientific NanoDrop® 2000 UV-Vis spectrophotometer combined with NanoDrop 2000 / 2000c Software
Plate reader
Tecan Infinite M200 Pro™ combined with Tecan iControl TM software
Absorbance measurments
Novaspec Plus™ Visible Spectrophotometer
UV- and dark hood
Biostep Dark-Hood DH-50™ combined with Argus-X1™ software and Biostep argusX1™ basic licence
Large Centrifuge
Eppendorf Centrifuge 5810 R
Small Centrifuge
Eppendorf Centrifuge 5424 R
Shaker
Kuhner Climo-Shaker ISF1-X
PCR machine
Applied Biosystems Veriti 96Well Thermal Cycler
Gel electrophoresis
Helixx Technologies MyRun®
Eppi-shaker/Heatingblock
Eppendorf Thermomixer compact
Vortex
Scientific Industries VortexGenie®2
Balance
MettlerToledo XS4002S DeltaRange® Balance>
Incubator
Thermo Scientific Heraeus Incubator
FACS
Sterile hood