Team:London Biohackspace/protocols/ecoli-tansformation
1. Prepare overnight cultures (for use ~8 hours later)Protocols
Electroporation Transformation of E. coli DH5α
1.1 Take 2 new/sterile 50ml falcon tubes and add to each 10ml of sterile LB broth (there is usually an autoclaved 1 L duran flask of LB in the fridge, use 20ml from that and transfer to tubes under the flow hood).
1.2 Inoculate the 2 falcon tubes from DH5α stock using a flamed loop.
1.3 Incubate at 37 °C with shaking overnight, or for 8 hours.
