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Under construction...

Anti-freeze protein

The antifreeze protein used in this project is normally produced by an insect, the Spruce Budworm, Choristoneura fumiferana[5]. The structure of the antifreeze protein is composed of beta-sheets stacked parallely. From a cross section the protein appears triangular with rectangular sides[6] (Fig. 1).

Resveratrol

Resveratrol is an antioxidant. Resveratrol is synthesized from p-Coumaroyl-CoA by stilbene synthase (STS) making the final enzyme in the resveratrol biosynthesis. Moss already produces the precursor p-Coumaroyl-CoA, which makes it simple to engineer moss to produce resveratrol.

Constructs

The construct for introduction and expression of the anto-freeze protein or STS contains are flanked by regions homologous to the moss genome so that the STS-gene will integrate by homologous recombination in moss. The constructs furthermore contains the ZmUbi-promoter, a kanamycin resistance gene and YFP, to confirm transformation. Three pieces are amplified by PCR and assembled by homologous recombination in moss. Click on the construct to learn more about the different parts. (Works only for the lower construct so far...).