Team:EPF Lausanne/Attributions

EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

Attributions

Team attributions

Experiments were designed and conducted by Emilie Cuillery, Paola Malsot, Rocco Meli, Ari Sarfatis, Victor Steininger, Grégoire Thouvenin and Nina van Tiel in E. Coli and by Joseph Heng, Vincent Jacquot, Cyril Pulver, Loïc Steiner and Axel Uran in yeast.

The initial idea for Bio LOGIC came from Ari Sarfatis.

The wiki was designed by Axel Uran. All teams members contributed to the content of the wiki.

The establishment of the model of the project was done by Paola Malsot, Rocco Meli, Cyril Pulver, Ari Sarfatis and Grégoire Thouvenin. The software implementation was done by Rocco Meli and Ari Sarfatis.

The Interlab study was conducted by Victor Steininger.

The design of our logos was done by Emilie Cuillery.

Aknowledgements

We would like to aknowledge all the help and support we received throughout the project and would like to thank everyone who helped in any possible way and together made our project possible.

General support

We would like to thank André Pexieder for letting us use his lab and its equipment during the project, Marie-France Radigois who helped us with the administrative tasks related to the project and Stéphane Karlen, Safety coordinator at the School of Life Science, who gave us a course on laboratory safety before our project started and was available for any question during the project.

Lab support

Special thanks to David Bikard, from the Institut Pasteur, for sending us E. Coli strains JEN202, JEN202+pWJ89 and DH5a+pWJ66, a central part for the wet lab in E. Coli. We would like to thank Miguel Garcia who taught us how to use the flow cytometer and the EPFL Flow Cytometry Core Facility for letting us use their flow cytometer. We would also like to thank Marc Chambon and Henrike Niederholtmeyer who taught us how to use the plate reader and Sebastian Maerkl for letting us use the plate reader in his lab. And thanks to Julie Russeil who helped us through our first successful yeast integration and gave us usefull tips for working with yeast.

Project advisor support

We would like to thank our advisors, Riccardo Dainese and Michael Frochaux, pHD students in Bart Deplancke's lab, and Ted Baldwin, Romane Breysse, Nikolaus Huwiler, Sakura Nussbaum and Cécile Piot, EPFL iGEM 2014 team members. They helped us get started in the lab and were available to answer any questions or help us out during the project. Special thanks to our instructors, Bart Deplancke, Barbara Grisoni and Sebastian Maerkl, for helping us and advising us throughout the project.

Project advisor support

Special tanks to Paolo De Los Rios, head of the Laboratory of Statistical Biophysics, for a very useful discussion on our model and for the advices and new inputs he gave us.

Human Practices support

Sponsors

We would like to thank all our generous sponsors.

EPFL

EPFL is one of the two Swiss Federal Institutes of Technology and is located in Lausanne, Switzerland. This school was founded with the stated mission to educate engineers and scientists, to be a national center of excellence in science and technology and to provide a hub for interaction between the scientific community and industry. It is our main sponsor.

F. Hoffmann-La Roche is a Swiss global health-care compagny hearquartered in Basel, Switzerland, that operates worldwide under two divisions : Pharmaceuticals and Diagnostics.

Nikon

Nikon is a Japanese multinational corporation headquartered in Tokyo, Japan, specializing in optics and imaging products, including cameras, camera lenses, binoculars, microscopes, ophtalmic lenses, measurement instruments, etc.

SnapGene is a molecular biology software that offers a fast and easy way to plan, visualize and document molecular biology procedures.

SnapGene
Microsynth

Microsynth is a European leader in offering services related to synthesis of DNA/RNA oligonucleotides, various kinds of DNA/RNA analyses and molecular biology project outsourcing.

Novartis is a Swiss multinational pharmaceutical compagny based in Basel, Switzerland, ranking number one in sales amog the world-wide industry in 2013.

Novartis
New England Biolabs

New England Biolabs Description coming soon

Integrated DNA Technologies (IDT) is a leader in development and manufacturing of products for research and diagnostic life science markets. It is the world’s largest supplier of custom nucleic acids and serves academic research, biotechnology, and pharmaceutical development communities.

Integrated DNA Technologies
EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits