1. Record
1.1 Plasmid Part
1.1.1 JUNE -- Week 1~4 -- June 6~25[Expand]
1.1.1.1 Week1 -- June 6
June 6
1, Design the PCR primers of chR2-YFP.
1.1.1.2 Week2 -- June 8~14
June 8
1,Theamplification of CHR2-YFP (use taq PCR protocol)
2, AGE ( agarose gel electrophoresis )
3, Gel extraction of chR2-YFP.
4, Transformation of GFP, YFP, mcherry in E•coli DH5α.
June 9
1, Select a single clone of each plate. (GFP, YFP, mcherry) .Put the E.coli in 4ml LB buffer and train for one night at 37℃.
2, Transformationof vector with pmyo-3(ppd95.77)
1.1.1.3 Week3 -- June 15~21
1.1.1.4 Week4 -- June 22~25
1.1.2 JULY -- Week 2~5 -- July 11~30[Expand]
1.1.2.1 Week2 -- July 11~14
1.1.2.2 Week3 -- July 15~21
1.1.2.3 Week4 -- July 21~27
1.1.2.4 Week5 -- July 28~31
1.1.3 AUGUST -- Week 1~5 -- August 1~31[Expand]
1.1.3.1 Week1 -- August 1~7
1.1.3.2 Week2 -- August 8~13
1.1.3.3 Week3 -- August 15~21
1.1.3.4 Week4 -- August 22~28
1.1.3.5 Week5 -- August 29~31
1.1.4 SEPTEMBER -- Week 1~2 -- September 1~10[Expand]
1.1.4.1 Week1 -- September 1~7
1.1.4.2 Week2 -- September 9~10
1.2 Worm Part
1.2.1 JUNE -- June 20~29[Expand]
1.2.1.1 June 20~29
1.2.2 JULY -- Week 2~5 -- July 9~31[Expand]
1.2.2.1 Week2 -- July 9~13
1.2.2.2 Week3 -- July 15~21
1.2.2.3 Week4 -- July 23~28
1.2.2.4 Week5 -- July 29~31
1.2.3 AUGUST -- Week 1~5 -- August 1~31[Expand]
1.2.3.1 Week1 -- August 1~6
1.2.3.2 Week2 -- August 8~14
1.2.3.3 Week3 -- August 15~21
1.2.3.4 Week4 -- August 22~27
1.2.3.5 Week5 -- August 29~31
1.2.4 SEPTEMBER -- Week 1~2 -- September 1~15[Expand]
1.2.4.1 Week1 -- September 1~7
1.2.4.2 Week2 -- September 9~15
1.3 Efficiency Testpart
1.3.1 AUGUST -- Week 1~5 -- August 3~31[Expand]
1.3.1.1 Week1 -- August 3~7
1.3.1.2 Week2 -- August 9~14
1.3.1.3 Week3 -- August 15~21
1.3.1.4 Week4 -- August 22~28
1.3.1.5 Week5 -- August 30
1.3.2 SEPTEMBER -- Week 1~2 -- September 1~15[Expand]
1.3.2.1 Week1 -- September 1~7
1.3.2.2 Week2 -- September 8~16
1.4 Equipment Part
1.4.1 JULY -- July 1,28[Expand]
1.4.1.1 Week1 -- July 1
1.4.1.2 Week4 -- July 28
1.4.2 AUGUST -- Week 1~2 -- August 1~8[Expand]
1.4.2.1 Week1 -- August 1~7
1.4.2.2 Week2 -- August 8
2. Timeline
