Team:EPF Lausanne/Interlab
Biologic Orthogonal GRNA-Implemented Circuit
In science, and in the field of synthetic biology in particular, characterizing new devices turns out to be as important as conceiving them. This not only provides a “how to use” guide for future users of your part but also allows the discovery of biologically relevant information about how it functions. When it comes to iGEM, the importance of characterization reaches huge proportion since thousands of new parts are registered each year. As a matter of fact, last year the competition launched its first InterLab Study, inviting every participating team to collaborate to measure previously existing devices. In addition to providing robust and statistically useful data, the InterLab Study aims at assessing how those measurements vary between labs. How similar are data from two teams using the same protocol? How well are the ratios conserved using two different measurement equipment? This year, these questions will be answered for the three constructs each team was given. They each contained a promoter from the widely used Anderson promoter collection that controlled the expression of a GFP. Each construct is described below. We contributed this year by measuring the three constructs in biological triplicates with a flow cytometer, which allowed us to assess the cell-to-cell variability of our samples. As part of the extra-credit assignment, we also provided technical triplicates of our data, thus determining the precision of the measurements.
Tested constructs
BBa_J23101
+
BBa_I13504
in
pSB1C3
Sequencing can be found here
BBa_J23101
+
BBa_I13504
in
pSB1C3
Sequencing can be found here
BBa_J23101
+
BBa_I13504
in
pSB1C3
Sequencing can be found here
Thinking Binary
Boolean Logic is the bedrock of the digital revolution. Developed by George Boole in the mid-19th century, it is based on a simple set of values: 0 (“FALSE”) or 1 (“TRUE”). Modern computers represent all forms of information using strings of the same 0s and 1s (also named “Bits”). The processing of bits is handled by logical transistors - which can be seen as electronically controllable switches. Elementary logic operation are performed using cleverly assembled transistors. Such assemblies are named “logic gates”. Gates are the bricks with which complex behaviour is produced.
