Team:UNITN-Trento/Experiments

Protocols for Characterization

  • Proteorhodopsin

  • BLH

  • PNCB

  • pMFC

Proteorhodopsin

Expression and Purification

Anaerobic growth

Proton-pumping measurements

Solar mimicking apparatus

Toxicity test by serial dilutions

ATP assay

15-15' Beta-carotene dioxygenase (BLH)

Cell growth

Extraction of pigments

TLC

UV-VIS

HPLC

Nicotinic Phosphoribosyl Transferase (pncB)

NAD/NADH quantification

pMFC

Berkley bacteria growth

pMFC data logging