15/07/07
Preparation of ONCs
Two times 5 mL LB were inoculated with TB43 and TB43 HU-mCherry and incubated at 37 °C and 200 rpm.
15/07/08
Preparation of Glycerol Stocks
Glycerol stocks of TB43 and TB43 HU-mCherry were prepared according to the glycerol stock generation protocol.
Preparation of electrocompetent Cells
Electrocompetent cells of TB43 and TB43 HU-mCherry were prepared according to the electrocompetent E.coli cells protocol.
Preparation of over-night culture (ONC)
Two times 5 mL LB were inoculated with TB43 and TB43 HU-mCherry and incubated at 37 °C and 200 rpm.
15/07/09
Preparation of electrocompetent Cells
Electrocompetent cells of TB43 and TB43 HU-mCherry were prepared according to the electrocompetent E.coli cells protocol.
15/07/10
Preparation of day culture
20 mL LB were inoculated with TB43 and TB43 HU-mCherry for flow cytometry and grown to an optical density (OD) of 0.5. No minicells could be detected.
15/07/11
Transformation
A GFP plasmid with Chloramphenicol resistance gene was transformed into TB43 and TB43 HU-mCherry via electroporation according to transformation into electrocompetent cells protocol.
15/07/13
Preparation of ONC
50 mL LB were inoculated with TB43 GFP and TB43 HU-mCherry GFP and incubated at 37 °C and 200 rpm.
15/07/15
Preparation of Minicells
TB43 GFP and TB43 HU-mCherry GFP were treated according to preparation of minicells protocol. Samples were taken and minicells could be detected via flow cytometry but only with very low yields.
15/07/27
Preparation of ONC
5 mL LB were inoculated with TB43, TB43 HU-mCherry, TB43 GFP and TB43 HU-mCherry GFP and incubated at 37 °C and 200 rpm.
15/07/28
Preparation of chemocompetent Cells
Chemocompetent cells of TB43, TB43 GFP, TB43 HU-mCherry and TB43 HU-mCherry GFP were prepared according to competent E.coli cells (RbCl) protocol.
15/08/21
Preparation of ONC
5 mL LB were inoculated with TB43, TB43 HU-mCherry, TB43 GFP and TB43 HU-mCherry GFP and incubated at 37 °C and 200 rpm.
Preparation of M9 Minimal Medium
Two media were prepared. One according to M9 minimal medium protocol and the other without glucose.
15/08/24
Plasmid Preparation
Cultures containing a plasmid which encodes enzymes for the biosynthesis of β-carotene were treated according to the Macherey-Nagel Plasmid DNA Purification Kit protocol.
15/08/25
Transformation
Carotinoid plasmids were transformed into chemocompetent TB43 and TB43 HU-mCherry according to Transformation into chemocompetent (RbCl) Cells protocol.
Plasmid Preparation
Cultures containing a plasmid which encodes enzymes for the biosynthesis of violacein were treated according to the Macherey-Nagel Plasmid DNA Purification Kit protocol.
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Nam liber tempor cum soluta nobis eleifend option congue nihil imperdiet doming id quod mazim placerat facer possim assum. Lorem ipsum dolor sit amet, consectetuer adipiscing elit, sed diam nonummy nibh euismod tincidunt ut laoreet dolore magna aliquam erat volutpat. Ut wisi enim ad minim veniam, quis nostrud exerci tation ullamcorper suscipit lobortis nisl ut aliquip ex ea commodo
