Team:China Tongji/Attributions

Contact with other teams to get communication with them, including NJAU, SYSU, SJTU, ITB and NYU. Also contact with Alexander Gottschalk’ lab in Goethe University, Frankfort, Germany to get their help.

Find sponsors: Contact with GENEWIZ and THORLABS to get us some project fund.

Help design of wiki page: Write basic material for our wiki page. Including protocol of our experiments, lab note, attribution and team introduction.

Travel arrangement.

4.1.3 Geyang Zhang

The art director: Design of our logo, team suit, banner, video, poster and postcard.

Test our C.elegans: Do the behavior test every day and maintain our worms. Including pmyo3-chR2, pmyo2-chR2, pmyo2-ic1c2, pmyo2-chETA, pmyo3-ic1c2, pmyo3-chETA, pttx3-chR2, pttx3-ic1c2 and ptwk16-chR2.

Modeling: Use the test result to build our optogenetics model.

Contact with Anna Moroni’s lab in University of Milan to get their blink plasmid.

4.1.4 Wenqi Cao

Idea maker: Finally came out of our project idea: optogenetic on C.elegan.

Build our parts: pmyo3-chR2 and pmyo2-chR2.

Microinjection: Tried pmyo-2-chR2 , pmyo2-blink, pmyo2-iC1C2, pmyo2-cheTA, pmyo-3-chR2, pmyo3-blink, pmyo3-ic1c2, pmyo3-chETA, pmec-3-chR2,cre-loxP system and pttx-3-chR2.

Paperwork: Experiment design. Write a part of background which include the mechanism of ChR2 and why we choose those opsins.

Travel arrangement.

4.1.5 Shan Qi

The equipment director: Assemble the light sources and improve their performances.

Test our C.elegans: Do the behavior test every day and maintain our worms. Including pmyo3-chR2, pmyo2-chR2, pmyo2-ic1c2, pmyo2-chETA, pmyo3-ic1c2, pmyo3-chETA, pttx3-chR2, pttx3-ic1c2 and ptwk16-chR2.

Modeling: Draw the C.elegans track and analyze them.

Paperwork: Experiment design.

4.1.6 Chenyu Pi

Make our fluorescence E.coli: Design the experiment and make out YFP, GFP and mcherryPet22b E.coli, which we are going to use in the traffic model of C.elegan. Then try to make YFP and GFP OP50 and PA14, these are the food of C.elegan.

Build our parts: pmyo2-blink, pmyo3-blink, pmyo2-ic1c2, pmyo2-chETA, pttx3-chR2, pttx3-ic1c2, pttx3-blink and pttc3-dsred.

Make our backbone: pSB1C3-pmyo3-chR2, pSB1C3-pmyo2-chR2, pSB1C3-pmyo3-blink, pSB1C3-pmyo2-blink, pSB1C3-pttx3-blink, pSB1C3-pttx3-chR2 and pSB1C3-pttx3-dsred.

Paint the plasmid profile.

4.1.7 Zikang Xing

Design our questionnaire and analyze the result.

Build our parts: pttx3-chR2, pttx3-blink, pttx3-ic1c2, pttx3-chETA and pttx3-dsred.

Update the pmyo2-dered.

Make our backbone: pSB1C3-pttx3-chR2, pSB1C3-pttx3-blink, pSB1C3-pttx3-ic1c2, pSB1C3-pttx3-chETA and pSB1C3-pttx3-dsred.

Point mutation: Update the pmyo2-dered.

Help with the modeling build.

4.1.8 Yini Yang

Design our questionnaire and analyze the result.

Build our parts: pttx3-chR2, pttx3-blink, pttx3-ic1c2, pttx3-chETA and pttx3-dsred.

Update the pmyo2-dered.

Make our backbone: pSB1C3-pttx3-chR2, pSB1C3-pttx3-blink, pSB1C3-pttx3-ic1c2, pSB1C3-pttx3-chETA and pSB1C3-pttx3-dsred.

Paper work: Human practice and the standards of the different medals.

Primer design all the tradition method of ligation’s primers.

Help contact with some biology company in order to get some funding.

4.1.9 Advisor Junwu Li

Help us make our wiki, which allows us to present out project in a beautiful way.

Give us some advice about the data organization and formal paper work.

4.1.10 Advisor Yuanyuan Wu

Teach us how to do the microinjection on C.elegan.

Help us make two of us plasmid: ptwk16-blink and ptwk16-ic1c2.

4.2 Acknowledgement

Here, we want to thank for all professors, teachers and advisors who have offered us great help during our project.

4.2.1 General support

First and foremost, we would like to show our deepest gratitude to our instructors: professor Songcheng Zhu and professor Wei Li. Professor Zhu, a respectable, responsible and resourceful scholar, who has provided us the advices of the project’s idea and molecular experiments. As an experienced instructor of C.elegan, Professor Li helped us a lot on the worm part. Dr. Li has also offered us many suggestions on our equipment assembling.

We also want to thank for our instructor MengHao, who is going to take us to the Jamboree!

4.2.2 Lab support

Here, we want to thank for all the labs which provided us with the experiment reagent, including professor Chao Zhang’s lab, professor Wei Li’s lab, professor Jianmin Fang’s lab, professor Jian Fei’s lab and professor Jiuhong Kang’s lab. We definitely couldn’t finish our project without their kind help!

4.2.3 Funding support

Thank for Professor Jing Zhang, who is in charge of our project in school. She helped us a lot in buying reagent and equipment.

Thanks for our college---Tongji University, they gave us enough funding to start our project.

Also, we want to thank GENEWIZ and THORLABS, these two companies are our project’s sponsors.

4.2.4 Wiki support

We are grateful to our advisor Junwu Li who helped us on designing our wiki page. Because of him, we can show our project in such a beautiful way!

4.2.5 Technique support

Our advisor Yuanyuan Wu taught us how to do the microinjection on C.elegans patiently. She also taught us how use the fluorescence microscope.

4.2.6 Human practice support

One of our human practice is exhibition at Shanghai Science Museum, thanks to NYU, they contacted with the museum so that we can have this fantastic human practice.

We have also made a popular science lecture at Tongji Primary School, thanks to Green Road Club which gave us this chance to let more young student know about synthetic biology.

4.2.7 Presentation coach

Professor Songcheng Zhu and professor Jing Zhang helped us on presentation coaching. Thanks for their patient instructions.

4.2.8 Plasmid support

Finally but no least, in our project, we used some other labs’ plasmids. Here we want to show our sincere thanks to these labs, which include Alexander Gottschalk’ lab in Goethe University, Frankfort, Germany and Anna Moroni’s lab in University of Milan, Milano. Thank you for your kindness!