Team:Hong Kong-CUHK/protocols
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(1) Bacterial DNA extraction protocol for Azotobacter vinelandii or E.coli
- We are using the TaKaRa MiniBest Bacteria Genomic DNA Extraction Kit of Takara according to the manufacurer directions.
(2) MiniPrep
- We are using the DNA-spin™ Plasmid DNA Purification Kit of Intron Technology according to the manufacturer directions.
(3) Preparation of chemically competent BL21 E.coli cells
Day 1
- Streak BL21 on a LB agar plate without antibiotic, grow overnight in 37℃ incubator
