Team:SPSingapore/Notebook-Week-2

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  • Week 1 (24/5 - 30/5)
  • Week 2 (31/5 - 6/6)
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Research Notebook

Week 2 (31/5 - 7/6) 31st May 2015 Xin Yi • Sent EsaR clone 4 and INv-4 for sequencing. 2nd June 2015 Yi Han • YFP and GFP transformation results - no colonies for YFP •The GFP transformation repeated with 100ng of plasmid was sucessful. • 4 colonies of gfp plasmid were inoculated in 3mL LB+amp and grown overnight. 3rd June 2015 Yi Han • Miniprep of gfp plasmids • RE digest with EcoRI and RsaI 4th June 2015 Chi Yan • RE digest indicated a very faint smaller band for colonies 2-4, and hence these were likely to be positive clones 5th June 2015 Yan Ting • Inoculated 3mLS of Inv-4 and EsaR-4 plasmid carrying bacteria into 100mL LB+ appropriate Antibiotic • Cell culture → HEK293 cells revived from freezing down appeared detached. 6th June 2015 Yi Han & Yan Ting • Storage of bacterial glycerol stocks for Inv-4, EsaR4 in 25% glycerol • Yan Ting: grew HEK293T cells in T25 glask • Gel extract to clean up gfp plasmids which loading dye had accidentally been added to.