Team:Marburg/InterLab
Aim
This year, the iGEM Team Marburg is participating together with many other teams in the InterLab Study. The aim of the InterLab Study is to investigating the reproducibility of BioBrick characterization by collecting data of the same experiment from iGEM Teams all around the globe. All teams are measuring the fluorescence of three different genetic devices constitutively expressing GFP who differ in their promoter strength. These data will allow a comparison of different protocols and lab techniques regarding the variety of the three parts. We transformed the three devices into E. coli cells, diluted to an optical density (OD) of 0.5 at 600 nm using both, a spectrophotometer and a plate reader and measured the fluorescence with a plate reader. The obtained results showed that the promoter J23101 was the strongest one, the promoter J23106 was weaker and the devices with promoter J23117 showed barely fluorescence over the background.
Advisors
- Daniel Hürtgen: Team Provide, Advised on experimental design, interpretation and presentation of results.
- Nicolas Koutsoubelis: Team Provide, Measurement, Advised on experimental design, interpretation and presentation of results.
- Anne Löchner: InterLab Study and Measurement Study, Advised on experimental design, interpretation and presentation of results.
- Max Mundt: Team Cut off, Advised on experimental design, interpretation and presentation of results.
- Oliver Schauer: Team Pick up, Advised on experimental design, interpretation and presentation of results.
- Daniel Schindler: Team Provide, Advised on experimental design, interpretation and presentation of results.
Parts and scientific support
- We thank the group of Prof. Wolfgang Hillen and Julia Bürger (Friedrich-Alexander-Universität Erlangen-Nürnberg) for sending us the tetR(B) and P5(L9A) promotors.
- We thank the group of Christopher S. Hayes (UC Santa Barbara) for providing us with the e.Coli strain EC93.
- We thank Dr. Knut Drescher (MPI Marburg) for helping isolating the DNA of the CDI-Operon.
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