Team:Tokyo Tech/Experiment/RNA thermometer assay
RNA thermometer assay
We have characterized previous parts.
contents
1. Introduction
2. Summary of the Experiment
3. Results
4. Discussion
5. Materials and Methods
5.1. Construction
5.2. Assay Protocol
5.2.1. Protocol1
5.2.2. Protocol2
6. Reference
1. Introduction
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2. Summary of the Experiment
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3. Results
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4. Discussion
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5. Materials and Methods
5.1. Construction
-Strain
All the samples were DH5alpha strain.
-Plasmids
Device 1: J23101 + I13504(pSB1C3)
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Fig.3-7-4-1. |
5.2. Assay Protocol
1. Prepare 2 over night cultures for each sample in 3 mL LB medium containing chloramphenicol (25 microg / mL) at 37 ºC for 12 h.
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6.. Reference
ここにコピペ。