Team:UFSCar-Brasil/part3.html

Part III

Frase de impacto

Curve fitting

Based on laboratory experiments was possible to plotting fluorescence standard curve as a function of GFP concentration (Green Fluorescent Protein). This adjustment was essential to facilitated the process of obtaining the GFP molecules number just using the fluorescence values obtained in lab.

The curve that better fitted the fluorescence standard curve points was the straight: (1) $$ F_{luo}=a[GFP]+b $$

Where the parameters a and b are constants, [GFP] is GFP concentrations given by nM and F_luo is fluorescence in u.a.

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