Team:Tokyo Tech/Project
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ssrA tag degradation assay
Replicating the Payoff Matrix
Overview of
fim
inversion system
FimB dependent
fim
switch state assay
FimE dependent
fim
switch state assay
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RNA thermometer assay
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contents
1. Introduction?
2.The original Prisoner’s Dilemma scenario in game theory
3. Replication of the payoff matrix using
E. coli
3.1 Prisoner
coli
’s dilemma payoff matrix
3.1.1 Both cooperation leads to low damage
3.1.2 Both defection leads to middle damage
3.1.3 When one is fooled, two types of damage
3.2 Modeling selected a solution for leaky expression problem to satisfy the requirement of the payoff matrix
3.3 Improvementof CmR protein property by addition of ssrA degradation tag
3.4 Succeeded to replicate the payoff matrix through wet lab
4. Prisoner
coli
decides its option : Cooperate or Defect
4.1 How to decide?
4.2 Decision making
coli
5. Iterated game with several strategies
5.1 The four strategies
5.1.1 Random strategy
5.1.2 Always cooperate strategy
5.1.3 Always defect strategy
5.1.4 Tit-for-tat strategy
5.1.4.1
Fim
E assay: recombinase that inverts fim switch in only one way
6. Dilemma game in general public
7. Reference
1. Introduction