Team:Tokyo Tech/Experiment/Overview of fim inversion system

Overview of fim inversion system

We introduce fim system

  
  

1. Introduction

      

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2. How to fim switch works

2.1. Wild type fim switch

2.1.1. The design of wild type fim switch

2.1.2. Result of FimB/FimE Assay to fimS(TT)

2.1.3. AHL dependent FimE Assay to fimS(TT)

2.2. Tokyo_Tech fim switch

2.2.1. The design of wild type fim switch

2.2.2. Result of FimB/FimE Assay to fimS(TT)

3. Comparison other team’s fim switch

3.1. FimB(2013_Toronto)

3.2. Fim switch(2006_Caltech)-FimE(2006_Caltech)

4. Reference

2.2. Wild type fim switch

3. Comparison other team’s fim switch

      

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4. Discussion

      

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5. Materials and Methods

5.1. Construction

-Strain

      

All the samples were DH5alpha strain.

-Plasmids

      

Device 1: J23101 + I13504(pSB1C3)

Fig.3-7-4-1.


5.2. Assay Protocol

5.2.1. Protocol1

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6.. Reference

      

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