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Transplanting KaiABC: Reduce your jet lag with this one weird trick!

Abstract

Currently, there exist no working 24-hour oscillator BioBricks. We are addressing this problem by transplanting the circadian rhythm-generating KaiABC system from cyanobacteria into E. coli and optimizing its function by controlling protein stoichiometry and adding accessory proteins. Such a BioBrick would be important for any application in which gene expression needs to be regulated on a 24-hour schedule. For example, it is often important to release a drug at a specific time of the day. As a proof-of-concept application, we implemented this clock as a potential solution to jet lag and sleep disorders by engineering a strain of E. coli that facilitates the production of a melatonin precursor in the gut on a 24-hour cycle. Thus, our project demonstrates the application of a robust KaiABC-based clock in timed drug dosage, as well as introduces an oscillator BioBrick into the synthetic biology community.

Welcome to iGEM 2015!

Your team has been approved and you are ready to start the iGEM season!

Before you start:

Please read the following pages:

• Requirements page
• Wiki Requirements page

Styling your wiki

You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.

While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.

Editing your wiki

On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!

Click here to edit this page!

See tips on how to edit your wiki on the Template Documentation page.

Templates

This year we have created templates for teams to use freely. More information on how to use and edit the templates can be found on the Template Documentation page.

Tips

This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:

• State your accomplishments! Tell people what you have achieved from the start.
• Be clear about what you are doing and how you plan to do this.
• You have a global audience! Consider the different backgrounds that your users come from.
• Make sure information is easy to find; nothing should be more than 3 clicks away.
• Avoid using very small fonts and low contrast colors; information should be easy to read.
• Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2015 calendar
• Have lots of fun!

Inspiration

You can also view other team wikis for inspiration! Here are some examples:

• 2014 SDU Denmark
• 2014 Aalto-Helsinki
• 2014 LMU-Munich
• 2014 Michigan
• 2014 ITESM-Guadalajara
• 2014 SCU-China

Uploading pictures and files

You can upload your pictures and files to the iGEM 2015 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name.
When you upload, set the "Destination Filename" to Team:YourOfficialTeamName/NameOfFile.jpg. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)

CLICK HERE TO UPLOAD FILES

Like our team Facebook page, Genehackers@UChicago!

Questions? Comments? Send us an email!