Team:Waterloo/Modeling/Cas9 Dynamics

Modelling Genomic Effects of CRISPR/Cas9

This page contains all our information about the Cas9 Dynamics Model.

Model Formation

Biology of Genomic Cutting, Repair and Deletions by CRISPR/Cas9

Assumptions

Pseudocode

Parameters

Talk about where all the probabilities come from

Results

Model Validation

Include notes on how the model matches reality/our expectations of reality in this section.

Simulate w/ targets that mismatch to different extents.

Effect of sgRNA Strength

Matt visualizations for different sgRNAs.

Graph of 3 different sgRNA designs of different strengths, show % functional

Importance of Large Deletions

Include notes on how the model matches reality/our expectations of reality in this section.

Effect of Cas9 Concentration

Include notes on how the model matches reality/our expectations of reality in this section.

Plant Defence by CRISPR/Cas9

Show % funcitonal for P6/time over many simulations.

Discussion

References

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