Mixed-Reality Cell

Bidirectinal coupling between real and virtual bio-oscillator

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Results

1. Here are results of per-experiment.

The growth curve of bacteria was s-shaped. The data of OD600 reached the top when cells were incubated for 11 hours and half of maximum for about 9 hours.

Fig.1 Growth curve of per-experiment

As is shown below, the maximum of this curve is about 0.133 at 16 hours and later then it begins to drop down. It is about half of maximum when sample was incubated about 9 hours, so we choose 9 hours as measuring situation.

2. Here are results of formal experiment

Each sample here was given in arbitrary units-fluorescence intensity/OD600.

We used 5ml centrifuge tubes to culture our samples and they were measured by a plate reader after 9 hours’ incubating. Three separate colonies were taken from the same sample.

The following is our calculation method.

F=F1-F0

O=O1-O0

F/O is the final data.

F1 : Fluorescence intensity of experimental groups(include three devices) .

F0 : Fluorescence intensity of negative control(E.coli with I13504 in pSB1C3).

O1: Optical density of experimental groups (include three devices) .

O0: Optical density of background(LB media).

Contacts

No.1, Shizishan Street, Hongshan District
430070,P.R.China
Email:hzauigem@gmail.com