Team:OUC-China
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Platform for Electromagnetic Control
how does it work?
This summer we designed Magthermo coli——a platform for remote regulation of gene expression by electromagnetic signal.
step 1
Our Magthermocoli contains 2 core compon-ents: magnetic receiver & thermosensitive regulator.
step 2
Inducing with electromagnetic field，magnetic receiver would be heated, thus raise the ambient temperature.
step 3
In response to the change of temperature, thermosensitive regulator would initiate GFP (or RFP) expression.
Magnetic Receiver
For magnetic receiver,we chose Ferritin, the iron-storage protein in organisms, which could synthesizeferric oxihydroxide core in its hollow protein shell. When exposed to electromagnetic field, the ferric oxihydroxide core would be heated, raising the ambient temperature.
In this session, we explored measurement techniques for the valuation of magnetism、biomineralization and Protein concentration per cell.
Thermosensitive Regulator
For thermosensitive regulator，we chose RNA thermometer and designed a thermosensitive T7 RNA polymerase.
RNA thermometer is a structured RNA which could expose RBS only at appropriate temperature. In this session, we explored the measurement method to identify the efficiency of thermosensitive regulator under heat stress.
Thermosensitive T7 RNA polymerase is a normal T7 RNA polymerase at whose selected locus was inserted by a temperature-sensitive intein, which could self-splice at specific temperature.
