Team:ETH Zurich/Basic Part

"What I cannot create I do not understand."
- Richard Feynmann

Basic Parts

In this section we present our basic parts. We expressed human Annexin V in Escherichia coli and then made a fusion protein with Annexin V and the Ice Nucleation Protein (INP). The former was created to allow our bacteria to serve as detectors of apoptosis by specifically binding phosphatidylserine.

Our other part is LldR, a protein required to regulate the lldR promoter. The expression of LldR was necessary to characterize the natural and our designed lldR promoters.

Part

Content

Registry number

Annexin V Promoter J23114-RBS B0034- Annexin V optimized for Escherichia coli BBa_K1847000
INP-Annexin V Promoter J23114-RBS B0034- INP-Annexin V fusion protein BBa_K1847015
LldR lldR BBa_K1847001
LldP-LldR lldP-lldR BBa_K1847001

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