Team:ETH Zurich/Basic Part

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• Modeling
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  • Lactate Module
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  • Compartment Model
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• About Us
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  • iGEM Team Roster

• Project
  • Project Description
  • Design
  • Applications
  • Achievements
  • Collaborations
  • Glossary
  • References
• Modeling
  • Overview
  • Lactate Module
  • AHL Module
  • Compartment Model
  • Reaction-Diffusion
  • Experiments Meet Modeling
  • Parameters
• Lab
  • Overview
  • Results
  • Chip Design
  • Experiments
  • Interlab Study
  • Safety
  • Notebook
  • Materials
• Human
  Practices
  • Overview
  • Medicine
  • Education
  • Awareness
  • Ethics
  • Business
  • Patents
• Parts
  • Overview
  • Basic
  • Composite
  • Part Collection
  • Characterization
• About Us
  • Team
  • Attributions
  • iGEM Team Roster

"What I cannot create I do not understand."
- Richard Feynmann

Basic Parts

In this section we present our basic parts. We expressed human Annexin V in Escherichia coli and then made a fusion protein with Annexin V and the Ice Nucleation Protein (INP). The former was created to allow our bacteria to serve as detectors of apoptosis by specifically binding phosphatidylserine.

Our other part is LldR, a protein required to regulate the lldR promoter. The expression of LldR was necessary to characterize the natural and our designed lldR promoters.

Part	Content	Registry number
Annexin V	Promoter J23114-RBS B0034- Annexin V optimized for Escherichia coli	BBa_K1847000
INP-Annexin V	Promoter J23114-RBS B0034- INP-Annexin V fusion protein	BBa_K1847015
LldR	lldR	BBa_K1847001
LldP-LldR	lldP-lldR	BBa_K1847001

We would like to thank our sponsors