Team:EPF Lausanne/Description
dCas9-ω
This part can be considered as an improvement of the biobrick dCas9-ω Activator (BBa_K1218014) . Our protein acts using sgRNAs (single guide RNA, such as BBa_K1723002) as guide RNAs [2], instead of tracrRNA/CRISPR array system. SgRNAs are more modular as they can be produced separately when needed in CRISPRi context. Also, the production of the complex is faster as less processing steps are needed. Finally, the sequences are shorter and facilitate the scalability.
