Team:London Biohackspace/protocols/filtercomp
One of the main backslashes of working in a community lab is the difficulty of long term storage of cells as buying and maintaining a -80 freezer is out of the budget of a small lab. We tested the long term storage of glycerol stocks of our constructs in -20, and with regular restreaking it's simple to maintain a library of plasmids in glycerol stocks. The main problem arose when we had to store competent cells, as they in fact very quickly loose competency (days-week).
pictures, table of colony count
Protocols
Filtration method for competent cells preparation
Introduction
The only way to have competent cells is to prepare them on the day, which slows down the work and is particularly time consuming. The protocol for preparing electrocompetent cells includes several washing steps, which, if carried out with a centrifuge with a standard methodology can take up to 2-3 hours, not including the preparatory work to initially grow the cells.
We approached this problem with a "hacker" spirit, and tried to
Materials and methods
Results
