Team:BroadRun-NorthernVA/Parts

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PART NAME	PART NUMBER
1. Promoterless alpha amylase with native secretion	BBa_K1871000
2. Alpha amylase with native secretion	BBa_K1871001
3. Alpha amylase with mating factor alpha secretion	BBa_K1871002

Composite Part 1: BBa_K1871000

Promoterless

Kozak sequence (Part BBa_K165002)

Native secretion sequence, from Bacillus amyloliquefaciens

Alpha amylase coding sequence from Bacillus amyloliquefaciens

ADH1 Terminator (Part BBa_K392003)

Composite Part 2: BBa_K1871001

cyc promoter (Part BBa_K105027)

Kozak sequence (Part BBa_K165002)

Native secretion sequence, from Bacillus amyloliquefaciens

Alpha amylase coding sequence from Bacillus amyloliquefaciens

ADH1 Terminator (Part BBa_K392003)

Composite Part 3: BBa_K1871002

cyc promoter (Part BBa_K105027)

Kozak sequence (Part BBa_K165002)

Native secretion sequence, from Bacillus amyloliquefaciens

Alpha amylase coding sequence from Bacillus amyloliquefaciens

ADH1 Terminator (Part BBa_K392003)