Team:UCSC/Field
Field
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Background:
Most cellulases found in nature cannot fold and catalyze properly in a high saline environment. H. volcanii and most halophiles can withstand environments with high concentrations of salt such as these salt flats in Fremont, CA. Microorganisms that produce cellulases can normally found on decaying plant matter or in the guts of animals that digest woody substances. |
Aims:
The aim was to produce butanol through this fermentation pathway. The focus was converting glucose to butanol by creating two fusion genes to complete the pathway from Butyryl Coa to Butanol. One from Shewanella and one consisting of native ACD and Aldy genes from HVO. In addition, we wished to grow multiple cultures both aerobically and anaerobically, and at different pHs, to test solvent production without modification. We were looking for butyric acid and/or butanol in our samples. |
Achievements:
We were able to create one assembled fusion gene. We also designed multiple linkers (refer to parts) to complete the designed fusion gene.
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| View our Notebook for our methodology that lead to our results. |
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Andre Baxter 
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"The iGEM program has been one of the most gratifying experiences of my life. Through it I was able to form a passion for research by developing an experiment from the ground up that grew alongside me during the summer. It's also a great feeling to actually contribute to the scientific community and potentially have a real impact on the world. I've gained far more than professional lab skills though, the relationships that I've built and the memories I've made will carry with me far into the future. In all, I've grown more in a few months than I have for years of undergraduate experience." |
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Ray Bryan 
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Tina DeLeon 
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"Although I have worked on teams before iGEM, I have never experienced such a sublime team chemistry. The Field team has shown me how to be patient when adjusting the pH of a solution, how to perform inoculations, and how to create cellulose growth medias (in addition to many more wet lab procedures). They have given me the strength to ask any and every question, no matter how obvious the answer may be. And they have given me confidence by showing support instead of criticism. The iGEM team as a whole has exposed me to synthetic biology, a field I have never studied. In addition to advancing my social skills in a lab setting, I have also been granted an opportunity to polish my bioinformatics skills. Overall iGEM has been an unforgettable experience and I cannot wait to continue this research in the upcoming school year. " |
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Adriana Landeros 
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"My experience with UCSC iGEM has been amazing! The exposure of doing field work and and working in lab has given me the confidence to continue developing my research experience as an undergrad. My favorite part of this summer has been sharing our project with the community. I've been motivated to bringing science presentations and college workshops to low income communities, such as my hometown Salinas where crime rate and gang activity is high. I feel we have the power to inspire the future innovators." |
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Sofia Menendez 
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"Participating in UCSC iGEM has been one of the most rewarding and life-changing experiences I have had to date. Prior to iGEM I had little hands-on experience in a laboratory setting, which was very structured and did not allow me to have the independence or freedom I craved in a lab. Since then, I have helped design protocols, learned different wet and dry lab techniques, and have gotten familiar with using different kinds of laboratory equipment. iGEM has given me such a different and unconventional lab experience; it has given me the confidence and leadership skills to attend meet-ups, give oral presentations, take my laboratory skills to a whole other level, and motivate me to follow my passions in science. All in all, iGEM has given me more than I would have ever expected and I am so glad I took advantage of all the opportunities and valuable well-rounded skills iGEM offered me" |
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Henry Vilas 
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Jocelyn Simlick 
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