Team:HZAU-China/WetLab/Characterization

Mixed-Reality CellBidirectinal coupling between real and virtual bio-oscillator

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Characterization

To ensure that our system of the real part can work as we expect and we can achieve our goal, we did some verifications and tests.

1、 the verification of oscillator

2、 the effect of light on the expression of genes

We verified the effect of light on the expression of genes by controlling the light condition of E.coli.

Two sample were set in our test experiment, the one in dark, the other in red light, and each sample had three repeats. Besides we designed a simple and practical device to control the light condition of E.coli as the following picture.

After some times gropes and tests, we gained related data and did correlation analysis as showing from the following table. As we can see from it, the presence of red light can inhibit the expression of target genes.(In the test, we used GFP as the target gene)

Fig. The effect of red light on the expression of genes.

3、 the test of the related promoter

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