Team:China Tongji/Achivement

Parts name	Type	Description	Length
BBa_K1634007	Composite	pmyo2-ChR2-YFP	2509
BBa_K1634008	Composite	Pmyo2-dsRed	1378
BBa_K1634004	Composite	pmyo3-ChR2-YFP ( C.elegans )	2629
BBa_K1634002	regulatory	pmyo2 ( a promoter in C.elegans )	694
BBa_K1634003	coding	ChR2-YFP	1809
BBa_K1634005	regulatory	pmyo3 (a promoter in C.elegans)	814
BBa_K1634006	Reporter	dsRed ( a reporter which is a red fluorescent protein)	678

Move your mouse on to the part line, you may see the sketch map below!

2. Equipment--Install our LED light Source

2.1 Introduction

ChR2 need a high power light to let it work on C.elegan. However, in our lab, we don’t have the light source which is powerful enough to let us use it for optogenetics. So we bought some parts from THORLABS, and prepare to build our own LED light source by ourselves.

Here are all the parts we need to build LED light source:

ACL2520-A

B1CM

B4CM

C4W

DC2100

DMLP550R

FFM1

FL460-10

FL532-10

FL560-10

FL635-10

LEDD1B

M470L3

M530L3

M590L3

M625L3

SM1A14

SM1CP2

SM1L03

SM1T2

SM1V05

TPS001

2.2 The installation of light source

(1) Put the proper light filter into SM1L03 and fix it by SM1RR, and then fix the SM1L03 containing its filter to its light source. After that fix ACL2520-A inside of SM1V05, then fix them in front of SM1L03. Link this packaged light source to the C4W cube by using SM1T2.

(2) Fix FFM1 to the B4C/M by using the prepared screws, then use FFM1 to nip the DMLP550R (please pay attention to the position which the filter located, the light whose wavelength is larger than 550nm will pass this filter, otherwise the light will be reflexed. When choosing the location of light sources, this matter should be considered as well). Then fixed the B4C/M to the cube carefully.

(3) The surface which fixed with B4C/M is the underside of the cube. Use B1C/M to seal up the top surface of the cube. Fix two proper light sources to the surfaces which lights come in of C4W. The surface which light comes out will links to the microscope by using SM1T2 and SM1A14. The remaining surface will blocked by SM1CP2.

(4) The installation has been completed.

Figure1: The LED light source
Figure2: We are trying to assemble C4W box of our light source
Figure3: We are figuring out how to assemble FFM1 into C4W box

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2.3 The adjustment of light path

(1) The light path will be collimated by changing the length between light source and convex lens. When the length is changing, pay attention to the light spot on the optical screen. If the spot is clear and convergent, it means the light path has been collimated.

(2) After join the light sources to the C4W cube, the light spot can be changed by rotating B4C/M and the screws on B4C/M.

(3) The adjustment has been completed.

Figure4: We are adjusting the light path, the spot is clear and convergent.

2.4 The refit of the light sources

After we build our LED light source, we found that it’s still not powerful enough for our experiment, so we decided to change the light source to 5W. we bought the 5W LED light from internet and replaced the old one.

(1) Take down the LED of the light sources by using welding gun. Replace them by the high power LED. Make sure the surfaces of the substrate and the radiator are parallel.

(2) The refit has been completed.

Figure5: The 5W red and green LED source which we bought from internet
Figure6: The 5W blue and yellow light source that we bought from internet
Figure7: We are trying to replace the old LED source with the 5W new one.

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3. Modeling

Cells sense the environment, process information, and make response to stimuli. To make cells work well in complex natural environments, lots of processes have to be preset to react to various signals. However, when well-characterized modules are combined to construct higher order systems, unpredictable behaviors often occur because of the interplay between modules. Another significant problem is that complex integrated systems composed of numerous parts may cause cell overload.

Figure 2. China Tongji logo

Our design approach may lead to a revolutionary step towards system integration in synthetic biology. Potential fields of application include organism development, living therapeutics and environment improvement.

4. Self Judgement

Cells sense the environment, process information, and make response to stimuli. To make cells work well in complex natural environments, lots of processes have to be preset to react to various signals. However, when well-characterized modules are combined to construct higher order systems, unpredictable behaviors often occur because of the interplay between modules. Another significant problem is that complex integrated systems composed of numerous parts may cause cell overload.

Figure 2. China_Tongji_iGEM_logo

Our design approach may lead to a revolutionary step towards system integration in synthetic biology. Potential fields of application include organism development, living therapeutics and environment improvement.

5. Judging Form

Click and link to the Team:China_Tongji/Judging Form!