Week 1 (24/5 - 30/5)
▪ June 14
Anaerobic Promoter
☀ Yi Han ☀
PCR
▶ RP_XhoI_GFP and FP_KpnI_GFP with GFP-3 as template
▶ for 7 reactions with control
Mastermix * 8 |
PCR buffer | 80ul |
Primers | 0.8ul/0.8ul |
DNA polymerase | 4ul |
dH2O | 494ul |
Templates | 38.75 |
Digest more plasmid (Esa plasmid)
4 rxns ( KpnI/XhoI digest) | 50ul each | Buffer | 20ul |
KpnI | 2ul |
XhoI 2ul | |
DNA | 34.8ul |
H20 | 141.2ul |
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