Team:Technion HS Israel/Project/Results

• Home
  Medal Fulfillment Home
• Project More about our project
  Description Results Parts
• Documents
  Experiments Notebook safety Protocols
• Modelling We take our math seriously
  Model Overview Plasmid Loss Equations Parameters Results Documents
• Software
  Software Overview Search Engine Constants Database Simulation About the Database
• Team We are a bunch of nerdy nerds
  Members Attributions and contributions Gallery
• Practices
  Collaborations Practices Search Engine Modeling Tutorials

Results and disscussions

characterize of YFP

characterize of YFP

characterize of YFP

In order to understand each module of our system, we started to test and characterize the first part of the genetic circuit. To do so, we built a BioBrick consisting of a constitutive promoter (BBa_I14032) driving expression of LuxR followed by a Lux promoter (BBa_R0062). The Lux promoter is inactive in absence of the AHL-LuxR complex and can be activated when AHL is present. Downstream of the pLux promoter we fused a gene encoding a florescent protein, in this case yellow fluorescent protein (YFP) (see Figure 1A).