Team:SPSingapore/Notebook-Week-6


Research Notebook

Week 6 (28/6 - 4/7)

▪ June 28

Lab Safety    ☀ SPSingapore Team ☀

BBPrefix_esaRBS PCR
H2O 221ul
Buffer 80ul
MgCl1 32ul
dNTP 32ul
FP_Bioricks Prefix 16ul
ORP_esaRBS_fragsyn 16ul
synpart_BBP_esaRBS 1ul
gotaq 2ul
Total 400ul
▶ Success-> PCR purification

esaRBS_GFP_BBsuffix
H2O 237ul
Buffer 80ul
MgCl2 32ul
dNTP 32ul
RP_BB_suffix 16ul
ORP_esaRS_GFP 16ul
plac_GFPPLASMID 1ul
gotaq 2ul
▶ Failed -> troubleshooting-> new OFP_esaRBBS_GFP
    Redid 30/6 with new primers
    Success with new primers-> fusion pcr


▪ June 29

Lab Safety    ☀ SPSingapore Team ☀

Safety Inspection for our lab by OSHE. We passed with flying colours!