Team:SPSingapore/Notebook-Week-6
Home
Team
Project
Protocol
Parts
Notebook
Human Practices
Safety
NOTEBOOK
PROTOCOL
ENTRIES
Week 1 (24/5 - 30/5)
Week 2 (31/5 - 6/6)
Week 3 (7/6 - 13/6)
Week 4 (14/6 - 20/6)
Week 5 (21/6 - 27/6)
Week 6 (28/6 - 4/7)
Week 7 (5/7 - 11/7)
Week 8 (12/7 - 18/7)
Week 9 (19/7 - 25/7)
Week 10 (26/7 - 1/8)
Week 11 (2/8 - 8/8)
Week 12 (9/8 - 15/8)
Week 13 (16/8 - 22/8)
Week 14 (23/8 - 29/8)
Week 15 (30/8 - 5/9)
Week 16 (6/9 - 12/9)
Week 17 (13/8 - 17/9)
Research Notebook
Week 6 (28/6 - 4/7)
▪ June 28
Lab Safety
☀ SPSingapore Team ☀
BBPrefix_esaRBS PCR
H2O
221ul
Buffer
80ul
MgCl1
32ul
dNTP
32ul
FP_Bioricks Prefix
16ul
ORP_esaRBS_fragsyn
16ul
synpart_BBP_esaRBS
1ul
gotaq
2ul
Total
400ul
▶ Success-> PCR purification
esaRBS_GFP_BBsuffix
H2O
237ul
Buffer
80ul
MgCl2
32ul
dNTP
32ul
RP_BB_suffix
16ul
ORP_esaRS_GFP
16ul
plac_GFPPLASMID
1ul
gotaq
2ul
▶ Failed -> troubleshooting-> new OFP_esaRBBS_GFP
Redid 30/6 with new primers
Success with new primers-> fusion pcr
▪ June 29
Lab Safety
☀ SPSingapore Team ☀
Safety Inspection for our lab by OSHE. We passed with flying colours!