Team:Marburg/Minicells

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Aim

Project Design

Results

Outlook

Background

Aim

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Project Design

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Results

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Outlook

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Background

Bacterial minicells are produced by asymmetric cell division, which is caused by a knock out of the minCDE-system. Naturally, this system regulates symmetric cell division.[1] The Min-proteins bind to the cell poles and oscillate between them. MinC functions as inhibitor for FtsZ, which forms the Z-ring and ultimately initiates division of the cell. This symmetric division ensures that each cell gets a copy of the chromosome and all other cell components and thus, is able to live on its own. If this system is disturbed, cell division occurs randomly leading to the formation of minicells.

Figure X: Normal functional min-system

As only a MinC knock down is needed to get minicells, we looked for a simple and modular solution. The knock down of gene expression through an small regulartory RNA (sRNA) system was already shown in iGEM by the Team Paris Bettencourt 2013 to was a great success for knock downs of target genes and was based on the Na et al. 2013 publication. The sRNA is processed by the Hfq protein and can then bind to the mRNA where it induces its degradation and stops the protein biosynthesis. Our system was designed according to the guidelines of the paper: a sRNA that binds with 24bp to the MinC mRNA (Figure X).

Figure X: Normal functional min-system

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