Team:Marburg/Minicells
Aim
Within NUTRInity-Provide, we aim to establish a shuttle system with countless applications. In our case, we want to produce micronutrients directly where the absorption in the human body takes place – in the small intestine. However, we do not want to release genetically modified microorganisms into our guts if it is not really necessary. Therefore, we want to find a system that can produce valuable compounds but is no longer able to divide. For this purpose, we want to establish a minicell production E.coli chassis and show that we can produce micronutrients like β-carotene. Furthermore, we built a modular system to transform any bacterial chassis into a minicell producer.
Project Design
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Results
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Outlook
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Background
Bacterial minicells are produced by asymmetric cell division, which is caused by a knock out of the minCDE-system. Naturally, this system regulates symmetric cell division.[1] The Min-proteins bind to the cell poles and oscillate between them. MinC functions as inhibitor for FtsZ, which forms the Z-ring and ultimately initiates division of the cell. This symmetric division ensures that each cell gets a copy of the chromosome and all other cell components and thus, is able to live on its own. If this system is disturbed, cell division occurs randomly leading to the formation of minicells.
Previous applications of minicells include drug delivery systems for the treatment of tumor cells to avoid inflammatory reactions which occur often when the drug is injected intravenously.[3] Also, the usage in vaccines as antigen delivery systems were tested successfully.[4] We did not find any evidence for toxicity or unwanted immunoreactions. This led us to the conclusion that the system could be FDA approved in the future.
To prevent infections as far as possible, minicells have to be isolated from their parent cells for these medical applications. Over the last decades, several methods were introduced. Chronologically, the first used technique was density gradient centrifugation.[2] Because of limitations in sample sizes and low yields, antibiotic treatments of the minicell-producing strains were performed.[5] Many antibiotics, e.g. Penicillin, kill bacteria during cell division, which is why they do not affect minicells. Lately, the treatment with antibiotics was combined with filtration to increase purification efficiency.[1] In the future we will include aspects of those techniques in our purification methods to get less “normal” cells, when we are selecting for minicells.
