Team:Bielefeld-CeBiTec/Notebook/Miscellaneous
Miscellaneous
- fluorescence imaging
- we took lysate with sfGFP, lysis buffer and purified GFP
- We got a filter from light engineering from the women cultural center Bielefeld e.V. ("Frauenkulturzentrum Bielefeld e.V.") and put it in front of the smartphone.
- the picture showed great evidence for possible fluorescence imaging with the right filters
This Week we started with the iGEM 2015 Measurement Interlab Study:
- Heat shock transformation of BioBricks from the distribution:
- BBa_K823005 (Anderson promoter J23101)
- BBa_K823008 (Anderson promoter J23106)
- BBa_K823013 (Anderson promoter J23117)
- BBa_I13504 (GFP)
iGEM 2015 Measurement Interlab Study:
- Colonies from the BioBrick Transformation were used to inoculate over night cultures
- 5 ml LB-Medium + Cm (20 µg/ml), 37°C
- Mini-preps of KRX(BBa_K823005), KRX(BBa_K823008) and KRX(BBa_K823013)
- Plasmids stored at -20°C
- Second heat shock transformation of chem. competent KRX with BBa_I13504
- Colonies were used to inoculate over night cultures (5 ml LB + Cm20, 37°C)
iGEM 2015 Measurement Interlab Study:
- Mini-prep of KRX(BBa_I13504)
- Plasmid stored at -20°C