Team:Dundee/Part Collection

The Building Blocks of Our Project

Part Collection

Part:BBa_K1590000 (hHBA)

FluID- Blood Detection

Coding sequences for human Haemoglobin A. Forms a tetramer consisting of 2 A-chains and 2B-chains (See BBa_K1590001 for Haemboglobin B). Binds to Haptoglobin (BBa_K1590002). It is still being tested if Haptoglobin interacts with either single chain, or if it only binds to the complex.

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Part:BBa_K1590001(hHBB)

FluID- Blood Detection

Coding sequences for human Haemoglobin B. Forms a tetramer consisting of 2 A-chains and 2B-chains (See BBa_K1590000 for Haemboglobin A). Binds to Haptoglobin (BBa_K1590002). It is still being tested if Haptoglobin interacts with either single chain, or if it only binds to the complex.

Registry page for this part

Characterisation of Haemoglobin B

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Characterisation of haemoglobin beta following SEC (size exclusion chromatography). A) The sample of concentrated fractions containing haemoglobin beta from nickel affinity purification was loaded onto a SEC column and the protein was eluted. B) 10µl of each fraction corresponding to the two observed peaks was mixed with 10µl of laemmli buffer and loaded onto a SDS gel (12.5% acrylamide). The bands observable on the gel are in line with the expected size of haemoglobin beta - 16kDa. C) Western blotting was then carried out against an anti-his antibody to confirm the presence of hHHB- His.