Team:Tokyo Tech/Description
Description
contents
0. Summary
1. sstAのパート
2. RNA thermometer
2.1.“Background”for our characterization
2.2. Summary of the experiment
2.3. Result
3. Results
3.1. Arabinose-dependent FimB (wild-type) expression
3.2. FLA analysis
4. Discussion
5. Materials and Methods
5.1. Construction
5.2. Assay Protocol
5.2.1 Arabinose dependent FimB expression
5.2.2. FLA analysis
6. Reference
0. Summary
iGEM 2015 Tokyo_Tech improved the function of BBa_K395160 by submission of BBa_K1632020, and characterization of BBa_K1333309, to meet gold medal criteria.
Furthermore, we have improved characterization of another previously existing part, RNA thermometer (FourU) coding sequence plasmid (BBa_K1333309), constructed by iGEM 2014 SYSU-China by the following three points. (1) We measured the function of BBa_K1333309 at 42 ºC, which wasn’t confirmed by iGEM 2014 SYSU-China. (2) We clarified the processing of the background derived from Negative control. (3) We explicated that the measuring equipment was flow cytometer.
go to RNA thermometer.