Team:SZU China/Notebook
Notebook
Combine Biological Unnatural Amino Acid(UAA)Orthogonal
genectical system with logic "AND" gate gene circuit
to detect bladder cancer cells precisely
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To discuss the decision of team project: CANCER SNIPER BLADDER
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Finished the project system in shuttle vector
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Transfected the two plasmid system in 5637 and T24 bladder cancer cells,and got a good result in Rluc(with amber mutation). Moreover,we got the control experiments data in human bladder fiber epithelial normal cells(HFC).
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Improved the original BioBrick TERT (BBa_K1722001).
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In order to increase the expression of the system,we remoulded the system in three palsmid.
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We greeted Tito Jankowski visiting SZU-iGEM,who is the world's first bio hackerspaces BioCurious founder.
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Began to distribute questionnaires Onlinehttp://www.sojump.com/jq/5569949.aspx
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Transfected the three plasmid system in the T24 and 5637 bladder cancer cells,but its result was not so good.Therefore,we continued to use the two plasmid system.
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In order to increase the expression of the system,we remoulded the system in three palsmid.