Team:EPF Lausanne/Results
..
In order to build dCas9-controlled circuits, we aim to use dCas9-inducible synthetic promoters to mimic the behaviour of a transistor (Go take a look at our design (LINK) if you haven't !). To see whether such promoters could be used in wider-scale circuits, we identified and tested the following desirable properties :
- Transistor response : Is the transistor inducible? Is its output modulable allosterically
- Transistor mutability : Is it possible to mutate the dCas9-targeted sites on transistor-like promoters without altering their behaviour and output levels ? Would it be possible to make a family of homogenous transistors that can be regulated independently?
- Transistor orthogonality : is regulation of a transistor specific? Is there cross-talk? Could several transistors work simultaneously?
- Transistor chainability : Can transistors can be linked serially to allow for multiple levels of information processing ?
References
[1] Farzadfard, F., Perli, S. D., Lu, T. K. (2013). Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth. Biol., 2 (10), pp 604–613.
