Team:Bielefeld-CeBiTec/Organisms
Organisms
The favorite pets of a synthetic biologist
Escherichia coli
We used the following E. coli strains during our project:
- KRX (Source: Promega)
- Genotype: [F´, traD36, ΔompP, proA+B+, lacIq, Δ(lacZ)M15] ΔompT, endA1, recA1, gyrA96 (Nalr), thi-1, hsdR17 (rk–, mk+), e14– (McrA–), relA1, supE44, Δ(lac-proAB), Δ(rhaBAD)::T7 RNA polymerase.
- ER2566 (T7 Express Competent E. coli (High Efficiency), source: New England Biolabs)
- Genotype: F- λ- fhuA2 [lon] ompT lacZ::T7 gene 1 gal sulA11 Δ(mcrC-mrr)114::IS10 R(mcr-73::miniTn10-TetS)2 R(zgb-210::Tn10)(TetS) endA1 [dcm]
Bacillus subtilis
In our project we used B. subtilis strain 168 with genotype trpC2 for the GABA operon sequence. You can find further information about the origin of this strain in Zeigler et al.
Agrobacterium tumefaciens
We isolated the blc-operon from A. tumefaciens C58.1. For detailed information about this organism please be referred to Wood et al. 2001.
References
Wood, D. W.; Setubal, J. C.; Kaul, R.; Monks, D. E.; Kitajima, J. P.; Okura, V. K. et al. (2001): The genome of the natural genetic engineer Agrobacterium tumefaciens C58. In: Science (New York, N.Y.) 294 (5550), S. 2317–2323. DOI: 10.1126/science.1066804.
Zeigler, Daniel R.; Prágai, Zoltán; Rodriguez, Sabrina; Chevreux, Bastien; Muffler, Andrea; Albert, Thomas et al. (2008): The origins of 168, W23, and other Bacillus subtilis legacy strains. In: Journal of bacteriology 190 (21), S. 6983–6995. DOI: 10.1128/JB.00722-08.
