Team:HokkaidoU Japan/Composite Part
Composite Part
BBa_K1714000
Our favorite composite part is BBa_K1714000. This part has pBad/araC as promoter, RBS B0034 as RBS, Ag43 thanatin as α-domain as CDS, double terminator as termnator. We recommend this part because of its low toxicity for host cells in inactivate form and it secret thanatins by Asp-N treating. Secreted thanatins are toxic for bacteria. So by seeing this result, we can succeeded in difficult thanatin production by using inactivate form using Ag43. Comparing the toxicity for host cells and thanatins production.