Team:HokkaidoU Japan/Composite Part

• HOME
• MICROBUSTERS
  • Project Description
  • Ag43 Secretion System
  • Other Systems
  • Future Work
  • Modeling
• PARTS
  • Team Parts
  • Composite Part
• NOTEBOOK
  • Protocol
  • E. coli
  • L. casei
• HUMAN PRACTICES
• ABOUT US
  • Team
  • Attributions
  • Collaborations
  • Safety

Parts

• Team Parts
• Composite Part

Composite Part

BBa_K1714000

Our favorite composite part is BBa_K1714000
. This part has pBad/araC as a promoter, BBa_B0034
as an RBS, Ag43 thanatin as α-domain as a CDS, double terminator as a terminator. We recommend this part because of its low toxicity for host cells in inactivate form and it secret thanatins by Asp-N treating. Secreted thanatins are toxic for bacteria. So by seeing this result, we can succeeded in difficult thanatin production by using inactivate form using Ag43. Comparing the toxicity for host cells and thanatins production, we thought 1mer version is our best composite part.