Team:SCUT/Parts
Parts
Overview
An important aspect of the
iGEM competition is the use and creation of standard biological parts. According
this page, you can find a list of all parts that have been registered. Our team
in 2015 iGEM plan to send 4 parts. The detailed information can be seen in the
registries and our result page.
Parts
abstract
BBa_K1724000: The CadA promoter. CadA promoter is a
cadmium-sensitive promoter. Its repressor is MerR. In high concentration of
cadmium, the Cd2+ can rapidly bind with MerR and remove its inhibition of CadA
promoter. The reverse is the opposition.
BBa_K1724001: Curli, the first identified functional
amyloid fibres, are extracellular protein fibers produced by many enteric
bacteria including Escherichia coli and Salmonella species . The curli system
exhibits numerous features that make it an ideal platform for the type of
materials engineering by way of synthetic biology that we envision. First, as
the curli nanofibre is composed primarily from the self-assembly of one small
protein, it presents a tractable entry point towards creating a large diversity
of biofilm extracellular matrices with conventional genetic engineering
methods. Second, the functional amyloid fibres formed by CsgA are extremely
robust, being able to withstand boiling in detergents and extended incubation
in solvents, increasing their potential utility in harsh environments. Finally,
recent findings have shown that the curli system can be used to efficiently
export natively unfolded polypeptides and can be used in a broad and modular
way for the display of various functional peptides throughout the E. coli
biofilm. CsgA, the dominant proteinaceous component in E. coli biofilms, is
capable of self-polymerizing in vitro into β-sheet-rich amyloid fibers that
bind to the amyloid specific dye Congo red (CR), resulting in a red shift and
green birefringence under polarized light, and thioflavin T (ThT), leading to
increased fluorescence at certain wavelengths.
BBa_K1724002: The mercury –sensing regulatory
protein, MerR(wild type), which regulates mercury resistance operons in
Gram-negative bacteria, is subjected to directed evolution in an effect to
generate a MerR mutant that responds to Cadmium ion but not mercury.That is,
the MerR mutant is the cadmium-sensing regulatory protein. To get MerR mutant,
Oligonucleotide-directed mutagenesis is used to introduce random mutations into
the key metal-binding regions of MerR. Finally, Getting the generated
Cd-specific MerR mutants appears to be unique.
BBa_K1724003: MntA from lactobacillus plantarum
encodes a membrane protein which transports Cd2+ and Mn2+ into the cell
,saturated at around 50 uM ,it is highly specific ,show rapid accumulation ,and
can accumulate metal ions effectively in dilute solutions .MntA is a member of
the p-type family of adenosine triphosphatases (ATPases). Its transport
efficiency is affected by some factors such as other ion of high concentration
and temperature.
Comment: We didn't apply it in our project now
, it belongs to our future work.