Team:Bielefeld-CeBiTec/Primers

iGEM Bielefeld 2015


Primers

Name Sequence (5´ → 3´)
Standard primers
VF2 TGCCACCTGACGTCTAAGAA
VR ATTACCGCCTTTGAGTGAGC
Date rape drugs
BlcR_fwd ATGGGCCAAAGGGGCCAAGT
BlcR_rev CTAGTCTTTCTGCGATCGGTACCC
pSB1C3_BlcR_fwd CTTGGGTACCGATCGCAGAAAGACTAGTACTAGTAGCGGCCGCTGCAG
pSB1C3_BlcR_rev TTGACACACTTGGCCCCTTTGGCCCATCTAGTATTTCTCCTCTTTCTCTAG
BlcC_rev TTACGCGTAAAATTCGGGAGCCTTC
pro_Blc_fwd ATGCACCTTCCTTGACACACT
pro_Blc_rev CATCGACCTCTCCCGCATTAG
K516030proB_rev GGGCCAAGTGTGTCAAGGAAGGTGCATCTCTAGAAGCGGCCGCGAATTCC
K516030proB_fwd TGAACTCATATGCGGGAGAGGTCGATGGCTTCCTCCGAAGACGTTAT
Arsenic biosensor
Ars_1_fwd (Cm_fwd) CGGCATCAGCACCTTGTC
Ars_1_rev tatgacttaacgaatgtgtaagtgcagaggCGGATAAAACACATCTGAAAATTC
Ars_2_fwd cctctgcacttacacattcgttaagtcataGGTACTAGTAGCGGCC
Ars_2_rev (Cm_rev) TATACGCAAGGCGACAAG
gBlocks
GABA_B_pSB1C3_f ataagaaaataccgacagggggatattcacTACTAGTAGCGGCCGCTGC
GABA_B_pSB1C3_r cataaaaaaatcccagtcacaggtgattgaCTCTAGAAGCGGCCGCGA
GABA_R_pSB1C3_f tagttttcaaccctttcagcggaggagccatcgTACTAGTAGCGGCCGCTGC
GABA_R_pSB1C3_r ctgtggattaacacaccacctactttttaaCTCTAGAAGCGGCCGCGA
Trp_sy_pSB1C3_f aaagaagttcatcatcaccatcaccactaaTACTAGTAGCGGCCGCTGC
Trp_sy_pSB1C3_r tctcctctttccctatagtgagtcgtattaCTCTAGAAGCGGCCGCGA