Team:Bielefeld-CeBiTec/Media

Media

LB

• For 1 liter LB:
• 20 g LB powder
• Fill the bottle with deionized H₂O


• For 1 liter LB-plates:
• 18 g LB powder
• 16 g Select Agar
• Fill the bottle with deionized H₂O

SOC

• Add the following components for 900 ml of distilled H₂O:
• 20 g Trypton
• 5 g Bacto Yeast Extract
• 2 ml of 5 M NaCl
• 2.5 ml of 1 M KCl
• 10 ml of 1 M MgCl2
• 10 ml of 1 M MgSO4
• 20 ml of 1 M glucose

Buffers

50X TAE Stock Solution

• For each litre of solution:
• 242 g Tris Base (MW=121.1)
• 57.1 ml Glacial Acetic Acid
• 100 ml 0.5 M EDTA


• mix Tris with stir bar to dissolve in about 600 ml of ddH₂O.
• add the EDTA and Acetic Acid.
• bring final volume to 1 l with ddH20.
• store at room temperature.


• Note: Final (1x) working concentration:
• 0.04 M Tris - Acetate
• 0.001 M EDTA